Core--Transgenic Mice

动物核心将培育转基因小鼠，这些小鼠将被鉴定和表征，用于项目1：设计完全中和聚合的α链；项目二：SS患者和小鼠的MRl和NIRS；项目三：镰状细胞粘附；项目4：镰状血管闭塞：多效性、上位性、表观遗传学。我们的职能主要是协助调查人员对创始人及其后代进行标记、鉴定和记录。这些小鼠将包括只表达人类血红蛋白HbS的NY1KO小鼠

The animal core will breed transgenic mice which will be identified and characterized for use in Project 1:Design of alpha-chains that fully neutralize polymerization; Project 2: MRl and NIRS in SS patients and mice; Project 3: Sickle Cell Adhesion; and Project 4: Sickle vasocclusion: pleiotropism, epistasis, epigenetics. Our function will be mainly to provide assistance to investigators in the marking, identification, and record keeping of founders and their offspring. These mice will include the NY1KO mice that express exclusively human hemoglobins: HbS and three different levels of HbF; the BERK mice (originally described by Paszty et al) and BERK mice expressing HbF. Knockouts for iNOS, nNOS, vWF, and P-selectin will be bred into our sickle lines as will a variety of knockout and transgenic lines described in Project 4. Also included are mice that express exclusively HbC and exclusively HbA, as well as suitable control mice for all of the proposed experiments. This core also provides analytic services for other projects: protein identification and quantitation (HPLC, IEF, mass spec), red cell characterization (p50, intracellular polymerization, 2,3-DPG analysis), and red cell density distribution.