UCD MUTANT MOUSE REGIONAL RESOURCE CENTER

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。描述（由申请人提供）：这是第一份竞争性持续申请的重新提交，要求NIH/NCRR为UC Davis突变小鼠区域资源中心（UCD-MMRRC）未来5年提供支持。UCD-MMRRC的总体目标是作为MMRRC国家计划的一个组成部分，接受、维护和分发用于生物医学研究的突变小鼠菌株。MMRRC项目接受转基因、基因敲除和所有其他类型的突变小鼠系，捐赠者无需支付任何费用，并在spf再分化和冷冻保存后，分发遗传育种种群的种质。在许多情况下，表型验证的小鼠只收取一小笔费用，用于非商业的学术研究目的。UCD-MMRRC目前已经对bb0009000个突变株进行了普查，这些突变株要么是活跃繁殖的小鼠菌落，要么是冷冻胚胎或种质，要么是胚胎干细胞克隆。此外，UCD-MMRRC与其他3个区域mmrrc合作，作为一个卓越的实验室小鼠生物学研究和教学中心，为科学界提供突变小鼠生物学，菌落管理，传统和先进的基因分型和表型分析能力，临床和解剖病理学服务，菌株救援，传染病诊断和医疗保健等领域的专业知识。为了实现这一使命，本申请陈述了4个相互关联的特定目标，重点是继续维持资源中的突变小鼠系，接受新菌株进入资源，最终将转移到资源中的研究和开发项目，以提高其功能效率和能力，最后提供扩展和小鼠生物学教育和培训的机会。这些具体目标由UCD比较医学中心（CCM）， UCD小鼠生物学计划（UCD- mbp）以及医学院和兽医学院的密切联系的教师专业知识促进。这些关系允许利用MMRRC的支持，从而为MMRRC国家计划以及生物医学和行为科学研究界带来更多机会和利益。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This is a resubmission of the first competitive continuing application requesting NIH/NCRR support for the next 5 years of the UC Davis Mutant Mouse Regional Resource Center (UCD-MMRRC). The overall objective of the UCD-MMRRC is to serve as a component of the MMRRC National Program, to accept, maintain, and distribute mutant mouse strains for use in biomedical research. The MMRRC program accepts transgenics, knockouts, and all other kinds of mutant mouse lines at no cost to the donor, and after SPF-rederivation and cryopreservation, distributes breeding stock orgermplasm of genetically. In many cases phenotypically verified mice for a small fee to requesting investigators for non-commercial, academic research purposes only. The UCD-MMRRC currently has a census of >9000 mutant strains maintained as either actively breeding mouse colonies, frozen embryos or germplasm, and/or embryonic stem cell clones. Further, the UCD-MMRRC serves in a coordinated network with 3 other regional MMRRCs as a center of excellence engaged in research and teaching on the biology of the laboratory mouse, providing the scientific community with professional expertise in mutant mouse biology, colony management, conventional and advanced genotyping and phenotyping capabilities, clinical and anatomic pathology services, strain rescue, infectious disease diagnostics and health care, and other areas. In keeping with this mission, this application states 4 interconnected Specific Aims that focus on continuing maintenance of mutant mouse lines in the resource, acceptance of new lines into the resource, research and development projects that eventually will be transferred to the resource to improve its functional efficiency and capabilities, and finally to provide opportunities for outreach and education and training in mouse biology. These Specific Aims are facilitated by closely linked faculty expertise of the UCD Center for Comparative Medicine (CCM), the UCD Mouse Biology Program (UCD-MBP), and the Schools of Medicine and Veterinary Medicine. These relationships allow leveraging of MMRRC support, resulting in expanded opportunities for, and benefit to, the MMRRC National Program and the biomedical and behavioral scientific research community.