UCD MUTANT MOUSE REGIONAL RESOURCE CENTER

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 描述(由申请人提供)：本提案旨在竞争性地更新基础设施、人员和资源的资金，以支持加州大学戴维斯分校(UCD-MMRRC)的MMRRC。UCD-MMRRC与密苏里大学哥伦比亚分校和北卡罗来纳大学教堂山分校的两个中心以及杰克逊实验室的信息、协调和服务中心(ICSC)一起，作为MMRRC国家项目的分发档案和数据中心。自10年前成立以来，UCD-MMRRC一直是世界上主要的突变小鼠档案和分布库之一。我们是三个中心中最大的档案馆，拥有超过28,500个突变小鼠品系的库存，占系统中所有活群体、种质和/或胚胎干细胞的约98%。UCD-MMRRC已经完成了来自30个国家500个机构的2000多名调查人员的近6000份订单。我们在该系统的创新和资源开发方面也一直处于领先地位，推出了新的产品(例如，基因陷阱ES细胞)和启动了新的服务(例如，胚泡注射，ICSI)，为小鼠模型增加了科学价值，并提高了研究人员对MMRRC的利用。UCD-MMRRC利用其基础设施和专业知识，通过与美国国立卫生研究院、生物技术公司和几个组织签订合同，存档、分发、定制品种、基因型和表型1000‘S额外的ES细胞突变、BAC转基因、Cre、ENU诱导和其他突变小鼠收藏，从而扩大了其服务角色。此外，我们与中心和NIH项目工作人员合作，制定和实施了广告和营销战略、数据库管理和信息学应用程序、网站和在线资源、客户和用户服务、运营程序和政策，以及国家项目的共同治理。 公共卫生相关性(由申请者提供)：UCD-MMRRC在确保在研究实验室中创建的有价值的人类疾病、发育和行为异常的小鼠模型在生物医学科学界广泛共享方面发挥主要作用。作为地区性分布的资料库和分布档案，UCD-MMRRC提供专业知识、基础设施、资源和服务，以永久保存小鼠品系，保护它们免受灾难性损失，避免遗传和表型漂移，并防止病原体污染和疾病。UCD-MMRRC通过使整个研究界能够获得和使用这种有价值的小鼠模型，促进和促进人类疾病新的诊断、治疗和预防策略的发现。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. DESCRIPTION (provided by applicant): This proposal seeks to competitively renew funding for infrastructure, personnel, and resources in support of the MMRRC at the University of California (UC) Davis (UCD-MMRRC). The UCD-MMRRC, alongside 2 centers at the University of Missouri-Columbia and University of North Carolina-Chapel Hill, and an Informatics, Coordination, and Service Center (ICSC) at The Jackson Laboratory, serves as the distribution archives and data center for the MMRRC National Program. Since its inception 10 years ago, the UCD- MMRRC has been one of the primary mutant mouse archive and distribution repositories in the world. We are the largest archive of three centers, with an inventory of over 28,500 mutant mouse lines representing ~98% of all live colonies, germplasm, and/or embryonic stem (ES) cells in the system. The UCD-MMRRC has fulfilled nearly 6000 orders from more than 2000 investigators at 500 institutions in 30 countries. We have also been a leader in innovation and resource development for the system, introducing new products (e.g., gene trap ES cells) and initiating new services (e.g., blastocyst injection, ICSI) that have added scientific value to mouse models and enhanced utilization of the MMRRC by researchers. UCD-MMRRC has leveraged its infrastructure and expertise to extend its service role by contracting with NIH institutes, biotechnology companies, and several organizations to archive, distribute, custom breed, genotype, and phenotype 1000's of additional ES cell mutants, BAC-transgenic, CRE, ENU-induced, and other mutant mouse collections. In addition, we have worked with the Centers and NIH program staff and developed and implemented advertising and marketing strategies, database management and informatics applications, website and online resources, customer and user services, operating procedures and policies, and shared governance of the national program. PUBLIC HEALTH RELEVANCE (provided by applicant): The UCD-MMRRC serves a primary role in ensuring that valuable mouse models of human disease, development, and behavioral abnormalities created in research laboratories are shared broadly among the biomedical scientific community. As a regionally-distributed repository and distribution archive, the UCD- MMRRC provides expertise, infrastructure, resources and services to preserve mouse strains in perpetuity, protect them from catastrophic loss, avoid genetic and phenotypic drift, and prevent pathogenic contamination and disease. By enabling availability and access of such valuable mouse models to the entire research community, the UCD-MMRRC fosters and promotes the discovery of new diagnostics, treatments, and prevention strategies against human diseases.