CRYOPROBE ACCESSORY FOR A 600 MHZ NMR SPECTROMETER: AIDS

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：阿拉巴马大学伯明翰分校核磁共振共享设施的600 MHz核磁共振波谱仪(Bruker Avance-600)目前为校园内的教职员工提供了最高的操作场地。从需要高灵敏度检测的痕量化合物的表征到利用3D/4D-核磁共振光谱学确定蛋白质的三维结构，不同的研究小组正在利用它开展各种项目。为了显著提高该仪器的效率，以适应这些生物分子系统的研究项目，请求购买用于600 MHz核磁共振系统的三重共振反向CryoProbe附件。与传统的室温探头相比，该探头的灵敏度提高了300%至400%(取决于样品)，并将显著提高600 MHz核磁共振波谱仪的能力，以满足UAB园区的需求。随着灵敏度的显著提高，该仪器可以：(I)将同位素标记蛋白质的3D/4D-核磁共振测量的数据获取时间从几周或几个月减少到仅几天，(Ii)增加核磁共振样品的产量，(Iii)允许研究仅在低浓度或仅少量可溶的生物分子，(Iv)允许对寿命有限的蛋白质和化合物的结构研究，以及(V)允许表征在毫米波浓度下倾向于二聚但在低浓度时以单体为主的蛋白质的结构特征。这次600 MHz的CryoProbe升级将对由8名教员组成的主要用户组的计划产生重大影响，他们的研究项目涉及人类载脂蛋白A-L、干扰素-tau、心肌调节蛋白、钙调蛋白与HIV gp41的相互作用、噬菌体P22支架蛋白、药物/DNA复合体、从炭疽芽孢杆菌分离的表面低聚糖以及低浓度代谢中间产物的1H和ISC表征等研究项目。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The 600 MHz NMR Spectrometer (Bruker Avance-600) at the NMR Shared Facility of the University of Alabama at Birmingham currently provides the highest operating field available for the faculty members on the campus. It is being utilized by a diverse group of investigators working on projects ranging from the characterization of trace amounts of compounds requiring high sensitivity detection to the three dimensional structure determinations of proteins by 3D/4D-NMR spectroscopy. To significantly enhance the efficiency of this instrument in accommodating these research projects on biomolecular systems, a request is made for the purchase of a triple resonance inverse CryoProbe accessory for the 600 MHz NMR system. This probe provides a sensitivity enhancement of 300% to 400% (depending upon the sample) over the conventional room temperature probe, and will dramatically and significantly enhance the capabilities of the 600 MHz NMR spectrometer in meeting the needs of the UAB campus. With the significant improvement in sensitivity, the instrument can :(i) reduce data acquisition times for 3D/4D-NMR measurements on isotopically labeled proteins from several weeks or months to just a few days, (ii) increase the through-put of NMR samples, (iii) permit the study of biomolecules that are available only in low concentrations or only sparingly soluble, (iv) permit the structural study of proteins and compounds that have a limited life time, and (v) permit the structural characterization of proteins that tend to dimerize at mM concentrations but are predominantly monomeric at low concentrations. This 600 MHz CryoProbe upgrade will have a significant impact upon the programs of a major user group consisting of eight faculty members with research projects dealing with multidimensional NMR structural investigations on human apolipoprotein A-l, interferon-tau, cardiac muscle regulatory proteins, interaction of calmodulin with HIV gp41, bacteriophage P22 scaffolding protein, drug/DNA complexes, surface oligosaccahrides isolated from the Bacillus anthracis Exosporium, and the 1H and ISC-characterization of metabolic intermediates in low concentration.