Core-Electron Spin Resonance

核心A的目的是为活性氧物种(ROS)和一氧化氮(NO)的最新测量提供帮助。在PPG的参与者的血管细胞和组织中。核心A将协助准备和处理各种生物样品，以便使用电子自旋共振(ESR)光谱进行分析。采用自旋捕捉技术的ESR光谱技术是最直接和最明确的方法之一，即使在强散射介质中也可以使用最少的生物材料来高灵敏度地定量检测活性氧物种。这个核心设施自1999年以来一直存在，并支持对超氧阴离子自由基(02.-)和NO的研究。在维管细胞上发表了几篇论文。 格里恩德林和哈里森(1胜10负)。这一设施将大大加强各个实验室的工作，并将明显改善PPG参与者之间的合作。 具体地说，电子自旋共振核心将： 1.为个人提供进行ROS和NO测量的专业知识、设施和培训。使用ESR波谱。 2.为ROS和NO的体外和体外测量提供帮助。在PPG参与者的血管细胞和组织中。 3.发展和完善现有的ROS和NO测定方法。在血管组织中。

The purpose of Core A is to provide assistance with state-of-the-art measurements of reactive oxygen species (ROS) and nitric oxide (NO.) in vascular cells and tissues for the participants of the PPG. CORE A will assist with preparation and processing of various biological samples for analysis using Electron Spin Resonance (ESR) spectroscopy. ESR spectroscopy with a spin trapping technique is one of the most direct and unequivocal methods for high sensitivity quantitative detection of reactive oxygen species even in strongly scattering media using minimal amounts of biological material. This Core facility has existed since 1999 and has supported studies of superoxide radical (02.-) and NO. in vascular cells in several publications from Drs. Griendling and Harrison (1- 10). This facility will significantly enhance the work of the individual labs and will clearly improve collaborations between participants of the PPG. Specifically, the Electron Spin Resonance Core will: 1. Offer expertise, facilities, and training for individuals in performing measurements of ROS and NO. using ESR spectroscopy. 2. Provide assistance with state-of-the-art in vitro and ex vivo measurements of ROS and NO. in vascular cells and tissues for participants of the PPG. 3. Develop techniques and refine existing methods for measurement of ROS and NO. in vascular tissues.