Apoptosis in the Generation of T-cell Memory

研究：这是一项研究计划，旨在研究细胞凋亡在T细胞记忆发育中的作用。 在感染后，T细胞经历快速克隆扩增，导致数量增加和更高 抗原特异性T细胞的频率。最初的扩张阶段紧跟在收缩阶段之后 (克隆性收缩)超过90%的抗原特异性T细胞被消除。幸存的人 抗原特异性T细胞创造了记忆池，并提供了持久的保护，防止再次挑战。这些研究 在此提出旨在阐明细胞凋亡具体塑造质量的机制 和发育中的抗原特异性T细胞记忆池的功能。我们的目标尤其是： 1)确定死亡受体介导的细胞凋亡途径在卵巢癌发生和动态平衡中的作用 记忆T细胞隔间。 2)检测Bcl2调控的线粒体依赖的细胞凋亡通路在 抗原特异性T细胞记忆的发育。 3)检测抗原介导过程中细胞增殖与细胞凋亡易感性的关系 免疫反应在T细胞记忆生成中的作用。 这项拟议的研究将我们对T细胞功能的理解与目前关于T细胞功能的观点结合在一起 细胞凋亡。候选人：本辛格博士以优异成绩毕业于宾夕法尼亚大学 2003年在宾夕法尼亚大学兽医学院获得博士学位。 在劳伦斯·图尔卡博士的指导下的医学。他在免疫学方面的论文集中在 调节性T细胞的发育和功能。他目前是La Jolla研究所的研究科学家 过敏和免疫学(L1AI)，道格拉斯·格林博士的实验室。*环境：格林博士是 现任LIAI细胞免疫学主任，被公认为是两个领域的专家 免疫学和细胞凋亡，有杰出的出版记录。他在训练方面有很好的记录 独立的科学家。LIAI是国际公认的免疫学和免疫领域的领导者 分子细胞生物学，并提供良好的环境和必要的内部设施进行 建议的研究。

Research: This is a research proposal to examine the role of apoptosis in the development of T cell memory. Upon infection, T cells undergo rapid clonal expansion resulting in both increased numbers and higher frequency of antigen specific T cells. An initial expansion phase is quickly followed by a contraction phase (clonal contraction) where more than 90% of the antigen specific T cells are eliminated. The surviving antigen specific T cells create the memory pool and provide durable protection from re-challenge. The studies proposed herein are designed to elucidate the mechanisms by which apoptosis specifically shapes the quality and function of the developing antigen specific T cell memory pool. In particular, our aims are to: 1) Determine the role of death receptor mediated apoptosis pathways in the generation and homeostasis of the memory T cellcompartment. 2) Examine the function of the Bcl-2 regulated, mitochondrial dependent apoptosis pathway in the development of the antigen specific T cell memory. 3) Examine the relationship between proliferation and susceptibility to apoptosis during antigen mediated immune responses in the generation of T cell memory. The proposed research integrates our understanding of T cell function with current ideas regarding the role of apoptosis. ^Candidate: Dr. Bensinger graduated summa cum laude from the University of Pennsylvania School of Veterinary Medicine and received his Ph.D. (2003) from University of Pennsylvania School of Medicine under the mentorship of Dr. Laurence Turka. His thesis in immunology was focused on the development and function of regulatory T cells. He is currently a research scientist at the La Jolla Instiute for Allergy and Immunology (L1AI) in the laboratory of Dr. Douglas Green. *Environment: Dr. Green is currently the head of cellular immunology at LIAI, and is recognized as an expert in both the fields of immunology and apoptosis with an outstanding publication record. He has a strong track record of training independent scientists. LIAI is an internationally recognized leader in both the fields of immunology and molecular cell biology and provides an excellent environment with the necessary in house facilities to conduct the proposed research.