Triple Quadrupole (Q-Trap) Mass Spectrometer

描述（由申请人提供）：纽约大学医学院Skirball研究所内的纽约大学蛋白质分析设施致力于确保生物医学科学家能够使用尖端的质谱技术来进行研究。我们正在申请资金用于三重四极杆（Q TRAP）质谱仪，以便能够对许多重要疾病中涉及的目标蛋白质和肽的数量进行特定和敏感的测量。这些靶向多重反应监测（MRM）实验将允许发现和验证蛋白质和肽生物标志物，用于早期检测疾病，如肺癌、卵巢癌、乳腺癌和前列腺癌，以及心血管和神经退行性疾病。此外，所要求的仪器将允许进行时程MRM实验，这将大大扩展用于研究神经元和其他细胞内信号转导的初步稳定同位素标记（SILAC）实验的发现。这些实验将大大增加我们对许多神经退行性、发育性和神经肌肉疾病以及糖尿病和HBV感染的原因和机制的理解。

DESCRIPTION (provided by applicant): The NYU Protein Analysis Facility within the Skirball Institute at the NYU School of Medicine is dedicated to ensuring that biomedical scientists have access to cutting edge mass spectrometry technology to enable their research. We are requesting funds for a triple quadrupole (Q TRAP) mass spectrometer to make possible the specific and sensitive measurements of quantities of target proteins and peptides involved in many important diseases. These targeted multiple reaction monitoring (MRM) experiments would allow for the discovery and verification of protein and peptide biomarkers for the early detection of diseases such as lung, ovarian, breast, and prostate cancers, as well as cardiovascular and neurodegenerative diseases. In addition, the requested instrument would enable time course MRM experiments that would greatly extend the findings of preliminary stable isotope labeling (SILAC) experiments for the study of intracellular signal transduction in neurons and other cells. These experiments would greatly increase our understanding of the causes and mechanisms of a number of neurodegenerative, developmental, and neuromuscular diseases, as well as diabetes and HBV infection.