LC-Triple Quadrupole Mass Spectrometer to Enhance LIfe Sciences Research

描述（由申请人提供）：要求使用现代化、最先进的三重四极杆质谱仪，配备在线HPLC功能、高通量自动进样器以及电喷雾电离（ESI）和大气压化学电离（APCI）源，用于定量和定性分析。该工具将成为支持10多个NIH资助项目的具体目标的重要组成部分。NIH资助的项目涉及各种分子种类的测量，包括脂质和磷脂、胆固醇、脂肪酸和甘油三酯、类固醇和肾上腺素、肽、核苷和核苷酸。通过使用三重四极杆分析仪的选择反应监测（SRM）和多反应监测（MRM），可以实现复杂基质的出色精密度和灵敏度检测限（LOD），这是大多数质量分析仪无法比拟的。使用三重四极杆分析仪，许多化合物的定量限（LOQ）可低于ng/mL（内标）。为了检测疾病的蛋白质生物标志物，三重四极杆MS的MRM功能允许在单个实验中以低变异系数（CV）同时监测多种蛋白质。因此，专注于发现疾病的新型蛋白质生物标志物的研究将受益于基于MRM的方法（富集特定靶点、低丰度肽）以验证候选或提议的生物标志物。拟议的LC-三重四极杆质谱仪系统将由加州大学洛杉矶分校分子仪器中心（MIC）支持和管理，该中心是一个校园范围内的综合设施，旨在提高现有共享的先进仪器设施对该机构更广泛的研究社区的可访问性。

DESCRIPTION (provided by applicant): A modern, state-of-the-art triple quadrupole mass spectrometer, equipped with on-line HPLC capabilities, a high-throughput autosampler, and electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) sources, for quantitative and qualitative analysis is requested. This instrument will be a vital component to support the specific aims of over 10 NIH-funded projects. The NIH-funded projects involve the measurement of a variety of molecular species, including lipids and phospholipids, cholesterol, fatty acids and triglycerides, steroids and prostaglandins, peptides, and nucleosides and nucleotides. With selected reaction monitoring (SRM) and multiple reaction monitoring (MRM) using the triple quadrupole analyzer, excellent precision and sensitive limits of detection (LOD) from complex matrices can be achieved that is unparalleled by most mass analyzers. Sub-ng/mL limits of quantification (LOQ) with internal standards can be achieved for many compounds using triple quadrupole analyzers. For detecting protein biomarkers of disease, the MRM functionality of the triple quadrupole MS allows for monitoring multiple proteins simultaneously with low coefficients of variation (CVs) in a single experiment. Thus, studies that focus on the discovery of novel protein biomarkers of disease would benefit from the MRM-based methodology (with enrichment of specific target, low abundance peptides) for validation of candidate or proposed biomarkers. The proposed LC-triple quadrupole mass spectrometer system will be supported and administered by the UCLA Molecular Instrumentation Center (MIC), a campus-wide, integrated facility formed to enhance the accessibility of existing shared, sophisticated instrumentation facilities to the broader research community at the institution.