QSTAR Elite Quadrupole-o-TOF LC/MS/MS System

描述（由申请人提供）：该NCRR共享仪器提案寻求购买应用生物系统/Sciex新的第五代QSTAR Elite(r)四极正交飞行时间质谱仪和相关的Tempo （Eksigent）纳米毛细管多维液相色谱（MDLC）站的资金。为了完成这个集成的LC MS/MS系统，我们还需要一个纳米离子源，以及ProteinPilot 2.0和MarkerView软件包。简而言之，我们建议将这种集成的LC MS/MS几乎完全用于复杂肽（和蛋白质）混合物的在线分离和分析，作为15名在最先进的蛋白质组学方面有重大需求的研究人员提供的24项NIH资助的大型小组的一部分。这种新仪器最关键的特点将是大大提高可以获得的串联质谱的数量（高达7 MS/MS谱/秒），总体灵敏度（低原子），动态范围（4 log），质量范围（5- 40000 m/z），碰撞能量，离子传输和定量软件功能。该仪器的独特功能将用于大量蛋白质和蛋白质组学应用，其中质谱法用于复杂混合物中的蛋白质/肽鉴定，相对定量，以及生物学上重要的酶和非酶翻译后修饰的测定，包括蛋白质磷酸化，氧化损伤和亚基化等。总体而言，该仪器的获得将大大提高16个项目的吞吐量、实验设计和发现率，这些项目正在研究人类健康的关键问题，包括衰老、神经退行性疾病、癌症、生物标志物发现、中风和传染病。

DESCRIPTION (provided by applicant): This NCRR shared instrumentation proposal seeks funding for the purchase of an Applied Biosystems/Sciex new 5th generation QSTAR Elite(r) quadrupole orthogonal time-of-flight mass spectrometer and associated Tempo (Eksigent) nanocapillary multidimensional liquid chromatography (MDLC) station. To complete this integrated LC MS/MS system, we are also asking for a nanospray ion source, as well as ProteinPilot 2.0 and MarkerView software packages. In brief, we propose to operate this integrated LC MS/MS almost exclusively for on-line separation and analysis of complex peptide (and protein) mixtures as part of large group of 24 NIH funded grants from 15 investigators who have significant needs in state-of-the-art proteomics. The most critical features of this new instrumentation will be to greatly improve on the number of tandem mass spectra that can be acquired (up to 7 MS/MS spectra/sec), overall sensitivity (low attomole), dynamic range (4 logs), mass range (5-40,000 m/z), collision energetics, ion transmission, and quantitation software features. The unique capabilities of this instrumentation will be directed at a large array of protein and proteomic applications where mass spectrometry is used for protein/peptide identification in complex mixtures, relative quantitation, as well as the determination of biologically important enzymatic and non-enzymatic posttranslational modifications, including protein phosphorylation, oxidative damage and nitrosylation, among others. Overall, the acquisition of this instrument will greatly improve the throughput, experimental design, and discovery rate of 16 projects that are examining key issues of human health, including aging, neurodegenerative disease, cancer, biomarker discovery, stroke and infectious diseases. The relevance of this new instrumentation to human health is significant, as 13 independent researchers at two institutions (Buck Institute and UCSF) will use this new technology to improve their ongoing studies in the basic biology of aging and age-related diseases, including Huntington's and Alzheimer's disease, Parkinson's disease, breast cancer, and stroke. In addition, a smaller group of four investigators at the Buck Institute, UCLA and University of Iowa, that are investigating pathogenic mechanisms in several human infectious diseases including tuberculosis, meningitis, genital ulcers, respiratory disease, tularemia, and otitis media.