Acquisition of Zeiss LSM 510 META confocal microscope

描述（由申请人提供）：我们正在申请购买蔡司LSM 510 Meta共聚焦显微镜的资金。获得所要求的最先进仪器将使本提案的用户能够进行活细胞和组织成像分析。拟议的激光扫描共聚焦显微镜将是一个重大升级，我们现有的光学显微镜设施的科学山校园耶鲁大学。本提案中指定的主要用户已经在使用光学成像技术来回答生物学中的各种基本问题。所要求的仪器将提高他们进行复杂和尖端图像分析的能力，这些分析是许多现代模式生物（如斑马鱼、果蝇、植物和小鼠）所需要的。这一工具促进的研究活动将包括先天免疫的调查（Dinesh-Kumar，主要研究者），细胞骨架和分子马达（Mark Mooseker，共同研究者），果蝇嗅觉系统（约翰·卡尔森），光调节发育开关（Xing-Wang Deng），神经嵴干细胞的起源和诱导（Martin Garcia-Castro），泛素/蛋白酶体介导的靶向蛋白水解（马克·霍赫斯特拉瑟），斑马鱼体节发生（斯科特霍利），突触发生的细胞和分子机制（Haig Keshishian）、细胞骨架动力学和细胞运动（托马斯波拉德），细胞内蛋白质与蛋白质及蛋白质与DNA相互作用的化学生物学（Alanna Schepartz）、轴突、树突和脊髓异常导致神经元发育缺陷和认知障碍（Elke Stein），学习和记忆（大卫威尔斯），以及神经发生和干细胞维持中的不对称细胞分裂（Weimin Zhong）。所要求的最先进的仪器无疑将对耶鲁校园科学山一侧的研究项目产生重大影响，填补了我们现有仪器的巨大空白。鉴于荧光显微镜在研究生物过程的动态和复杂性方面的当前和未来的中心地位，获得最先进的仪器将最大限度地发挥我们目前资助的NIH研究的影响。

DESCRIPTION (provided by applicant): We are requesting funds for the acquisition of a Zeiss LSM 510 META confocal microscope. Acquisition of the requested state-of-the-art instrument would allow users of this proposal to perform live cell and tissue imaging analyses. The proposed laser scanning confocal microscope will be a major upgrade to our existing light microscope facility on the Science Hill campus of Yale University. Principal users designated in this proposal are already using optical imaging techniques to answer a variety of basic, fundamental questions in biology. The requested instrument will enhance their ability to perform complex and cutting edge image analysis required by many of the modern model organisms like zebrafish, Drosophila, plants and mice. Research activities facilitated by this instrument will include investigation of innate immunity (Dinesh-Kumar, Principal Investigator), cytoskeleton and molecular motors (Mark Mooseker, co-investigator), Drosophila olfactory system (John Carlson), light regulated developmental switch (Xing-Wang Deng), origin and induction of neural crest stem cells (Martin Garcia-Castro), ubiquitin/proteasome-mediated targeted proteolysis (Mark Hochstrasser), zebrafish somitogenesis (Scott Holley), cellular and molecular mechanisms of synaptogenesis (Haig Keshishian), cytoskeletal dynamics and cellular motility (Thomas Pollard), chemical biology of protein-protein and protein-DNA interactions inside the cell (Alanna Schepartz), axonal, dendritic and spinal abnormalities leading to neuronal developmental defects and cognitive impairment (Elke Stein), learning and memory (David Wells), and asymmetric cell division in neurogenesis and stem-cell maintenance (Weimin Zhong). The requested state-of-the-art instrument undoubtedly will have a significant impact on the research programs on the Science Hill side of the Yale campus by filling a substantial void in our available instrumentation. Given the current and future centrality of fluorescence microscopy in studying the dynamics and complexity of biological processes, access to state-of-the-art instrument will maximize the impact of our currently funded NIH research.