A Strain Repository for Dictyostelium discoideum

描述(由申请人提供)：目前的申请是DictyostelisStock Center Repository赠款的延续。在最初的赠款期间，我们成功地开发了与Dictyostelius中央信息网站DictyBase的接口。我们已经回收、注释和存储了近900个菌株和200多个有用的质粒。研究界对这些产品的需求越来越大。我们提供了来自研究和教学社区的数据和信件，以支持这一资源的使用。在目前的应用中，我们建议增加菌株和质粒的收集，并解释为什么新的研究方向需要新的材料。我们建议完成遗产收藏的恢复、注释和存储，并纳入大规模基因敲除突变项目的产品。我们将通过与DictyBase的人员合作，使用为开放生物本体论开发的标准来描述每个突变的表型，从而使收集更加有用。我们将继续回收和注释可用于研究的质粒，包括整合和染色体外质粒，以及与GFP、YFP和其他标记融合的各种启动子的质粒。我们提供新制造的诱导型和cre-lox质粒。我们将向这些机构提供尽可能多的信息。最后，我们将以其他难以生产的试剂的形式添加新的成分，包括REMI文库、cDNA表达文库，以及当它们可用时，条形码突变体的集合。库存中心将继续提高研究效率，保障生物资源可用安全。

DESCRIPTION (provided by applicant): The current application is for a continuation of The Dictyostelium Stock Center Repository grant. During the initial grant period we have successfully developed an interface with dictyBase, the Dictyostelium central nformational website. We have recovered, annotated, and stored nearly 900 strains and more than 200 useful plasmids. These are in increasing demand by the research community. We provide data and letters from the research and teaching community supporting the utility of this resource. In the current application we propose to increase the collections of strains and plasmids and explain why new research directions demand new material. We propose to complete the recovery, annotation and storage of legacy collections and to incorporate the products of large-scale knockout mutagenesis projects. We will make the collections more useful by collaborating with the personnel of dictyBase to describe the phenotypes of each mutant using the standards developed for Open Biological Ontology. We will continue to recover and annotate plasmids that can be used for research, including integrating and extrachromosomal plasmids and plasmids with a variety of promoters fused to gfp, yfp and other markers. We provide newly made inducible and cre-lox plasmids. These will be provided with as much information as possible. Finally, we will add a new component in the form of other hard to produce reagents including REMI libraries, cDNA expression libraries, and, as they become available, collections of bar-coded mutants. The Stock Center will continue to increase the efficiency of research and to guarantee the security of availability of biological resources.