3D Digital Modeling of the Drosphila Brain

描述（由申请人提供）：数字3D模型在神经科学中发挥着越来越重要的作用。代表三维支架，其中功能数据和基因表达数据被输入和图形化显示，数字模型成为分析工具，允许人们解决神经连接和功能，以及基因功能和基因相互作用。这项拨款申请提议生成一系列标准的发育中的果蝇大脑数字图谱模型，这是一个被许多人用来研究控制神经元回路形成和功能的遗传机制的系统。苍蝇的大脑是由一组不变的神经母细胞谱系形成的，这些谱系代表了细胞体位置、轴突投射和（在一定程度上将在本提案中讨论）连通性方面的结构单位。轴突和树突状分枝，建立了从胚胎晚期到成年可见的形态学上不同的神经堆隔室。隔室和谱系形成了将在拟议的数字模型中捕获的模式化模式。考虑到它们对我们和其他人的有用性以及可行性，我们提出了以下模型：(1)早期胚胎神经母细胞图谱，(2)与进化的神经堆相关的胚胎晚期初级谱系，(3)与进化的神经堆室相关的幼虫晚期次级谱系，(4)进化的次级束系统和蛹的神经堆室。这些模型代表了一个完整的系列，因为每个神经母细胞的“遗传地址”，由早期胚胎神经母细胞图谱中表达的已知基因集定义，将与晚期胚胎、幼虫和蛹的神经元种群及其轴突模型相关联。这个建模项目的目标是提供一个与社区共享的工具，允许更有效地利用果蝇的大脑来研究发育-遗传和功能问题。神经谱系的模型将使表达特定的实验和解释突变表型成为可能。

DESCRIPTION (provided by applicant): Digital 3D models play an increasingly important role in neuroscience. Representing three-dimensional scaffolds in which functional data and gene expression data are entered and displayed graphically, the digital models become analytical tools that allow one to address neural connectivity and function, as well as gene function and gene interactions. This grant application proposes to generate a series of standardized digital atlas models of the developing Drosophila brain, a system used by many to investigate the genetic mechanism controlling the formation and function of neuronal circuits. The fly brain is formed by an invariant set of neuroblast lineages which represent structural units in terms of cell body location, axonal projection, and (to an extent that will be addressed in this proposal) connectivity. Axonal and dendritic arborizations, establish morphologically distinct neuropile compartments that are visible from the late embryo towards the adult. Compartments and lineages form a stereotyped pattern that will be captured in the proposed digital models. Having in mind their usefulness for us and others, as well as feasibility, the following models are proposed: (1) early embryonic neuroblast map, (2) late embryonic primary lineages in relation to evolving neuropile, (3) late larval secondary lineages in relation to neuropile compartments, (4) evolving secondary tract systems and neuropile compartments of the pupa. These models represent an integrated series because the "genetic address" of each neuroblast, defined by the known sets of genes expressed in the early embryonic neuroblast map, will be linked to the population of neurons and their axons modeled for the late embryo, larva and pupa. The goal of this modeling project is to provide a tool shared with the community, allowing to exploit the Drosphila brain more efficiently for developmental-genetic and functional questions. Models of neural lineages will make it possible to phrase specific experiments and to interpret mutant phenotypes.