Optical Imaging of S1 Cortex in the Awake Primate

描述（由申请人提供）：本研究提案的目标是开发用于清醒行为猴子的光学成像方法。光学成像在揭示麻醉动物的皮层组织方面非常成功。我们建议发展这种方法来研究皮层激活模式的体感生理反应的体感皮层在麻醉和清醒的猴子。然而，很少有人知道的空间模式的激活清醒，行为的动物。所提出的方法是一个非常新颖的方法来研究体感皮层功能。通过将高空间和时间分辨率的皮层激活模式图像与行为直接联系起来，它有望对皮层功能的观点产生很大影响。我们有两个目标。一个目标是开发一种能够同时具有高空间和时间分辨率的光学成像系统，用于清醒的行为猴子。内在信号和电压敏感染料成像能力将被纳入一个单一的系统。这涉及硬件和软件开发，在行为猴子中进行测试，以及开发新的数据分析方法。我们的第二个目标是在清醒的猴子中进行探索性的光学成像研究。麻醉和清醒状态下的SI在地形、功能组织和区域间激活方面存在显著差异。我们的目的是探索这些差异是否与注意力和任务依赖性上下文等因素相关。鉴于我们的初步数据，这些研究很可能会证明是富有成效的，并将成为未来R01在行为猴子中的基础。所提出的方法是新颖的，将允许一种新的方法来研究认知功能。此外，这种对行为猴子的功能成像将为单单位生理学、功能磁共振成像和行为研究提供一个急需的联系。

DESCRIPTION (provided by applicant): The goal of this research proposal is to develop the optical imaging methodology for use in awake, behaving monkeys. Optical imaging has been highly successful in revealing cortical organization in anesthetized animals. We propose to develop this method to study cortical activation patterns in somatosensory physiological responses of somatosensory cortex in anesthetized an awake monkeys. However, little is known about the spatial patterns of activation in awake, behaving animals. The proposed methodology is a highly novel approach to the study of somatosensory cortical function. By directly relating high spatial and temporal resolution images of cortical activation patterns to behavior, it promises to have a high impact on views of cortical function. We have two aims. One aim is to develop an optical imaging system capable of both high spatial and temporal resolution for use in awake, behaving monkeys. Both intrinsic signal and voltage sensitive dye imaging capabilities will be incorporated into a single system. This involves hardware and software development, testing in behaving monkeys, and development of new data analysis methods. Our second aim is to do exploratory optical imaging studies in awake monkeys performing tactile tasks. There are significant differences in topography, function organization, and inter-areal activations between anesthetized an awake SI. We aim to explore whether these differences correlate with factors such as attention and task- dependent context. Given our preliminary data, it is highly likely that these studies will prove fruitful and will form the basis for future R01s in behaving monkeys. The proposed methodology is novel and will permit a new approach to the study of cognitive functions. Further, this type of functional imaging in behaving monkeys will provide a much needed link between bodies of single-unit physiology, fMRI, and behavioral studies.