Fast Optical Imaging of Cortical Signals in the Behaving Primate

行为灵长类动物皮层信号的快速光学成像

• 批准号: 7139774
• 负责人: ANNA W. ROE
• 金额: $ 22.96万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-30 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7139774
• 关键词: Macaca; Primates; behavior; cognition; dyes; electrical potential; fasting; prefrontal lobe /cortex; short term memory; wakefulness

DESCRIPTION (provided by applicant): The goal of this research proposal is to develop a fast temporal resolution optical imaging methodology for use in awake, behaving monkeys. If successful, this methodology would provide online visualization of brain function at both high spatial (10's of ums) and high temporal (1-10 msec) resolution. Intense effort has been devoted to studying anatomical organization and physiological responses of sensory cortex in anesthetized and awake monkeys. However, little is known about the spatial patterns of activation in prefrontal cortex of awake, behaving animals during performance of working memory tasks. The goal is to observe clustered and distributed clusters of neuronal activation of cortical activity during behavior. These data would also provide a critical link between single-unit physiology, functional imaging studies, and behavioral studies. Importantly, the proposed technology development will provide new methodologies for studying spatial (functional organizational) and temporal aspects of cortical processing relating to vision, attention, memory, and cognitive function. Clinical relevance of data obtained from this approach would be relevant to cognitive dysfunctions in diseases such as schizophrenia. The specific aims of this proposal are: 1) development of fast optical imaging system for awake, behaving monkeys. This aim would include development of voltage sensitive dye methodology, noise reduction techniques for optical detection of neuronal activity, rapid image processing techniques suitable for large volumes of streamed data, and tandem electrophysiological recordings. 2) application of this method to study working memory processes in prefrontal cortex. Macaque monkeys would be trained on oculomotor delay response tasks. Delay period activity in prefrontal cortex would be imaged at high temporal (1-10 msec) to monitor changing spatiotemporal activity patterns depending on task demands. This methodology will provide a new approach for examining relationship of cortical organization with behavior, one that will complement the spatiotemporal capabilities of other existing technologies such as fMRI and single unit electrophysiology.

描述（由申请人提供）： 这项研究的目的是开发一种快速的时间分辨率的光学成像方法，用于清醒的，行为的猴子。如果成功，这种方法将提供高空间（10 μ s）和高时间（1-10毫秒）分辨率的脑功能在线可视化。人们一直致力于研究麻醉和清醒猴感觉皮层的解剖结构和生理反应。然而，很少有人知道的空间模式激活清醒的前额叶皮层，行为动物在工作记忆任务的性能。目的是观察行为过程中皮层活动的神经元激活的集群和分布式集群。这些数据也将提供一个重要的联系之间的单一单位生理学，功能成像研究，和行为研究。重要的是，拟议的技术开发将提供新的方法来研究与视觉，注意力，记忆和认知功能有关的皮层处理的空间（功能组织）和时间方面。从这种方法获得的数据的临床相关性将与精神分裂症等疾病的认知功能障碍有关。本项目的具体目标是：1）开发用于清醒、行为正常的猴子的快速光学成像系统。这一目标将包括开发电压敏感染料方法、用于神经元活动光学检测的降噪技术、适用于大量流式数据的快速图像处理技术以及串联电生理记录。2)应用该方法研究前额叶皮层的工作记忆过程。猕猴将接受眼部延迟反应任务的训练。前额叶皮层的延迟期活动将在高时间（1-10毫秒）成像，以监测根据任务需求变化的时空活动模式。这种方法将提供一种新的方法来研究皮层组织与行为的关系，这将补充其他现有技术，如功能磁共振成像和单单位电生理学的时空能力。