Sample Distribution, Processing and Culture Core

有效的组织采购和利用对于有意义的翻译研究活动至关重要。《核心》 将与每个AML-P01项目以及生物统计和数据管理核心合作，以确保高效 以及高度协调的患者衍生材料的采购、使用和存储。 样本分发和处理部分A部分核心有以下目标： 1.建立和维护血液和骨髓标本的储存库，包括完整的细胞、血清、 血液病患者的细胞DNA、RNA和蛋白质，包括 在M.D.安德森癌症接受治疗或评估的新诊断、缓解和/或复发中 中心。将组织标本分发给AML-P01调查人员进行分析，并提供 对AML-P01项目中对组织切片进行的研究的解释提供了全面的 AML-P01项目中使用的血液和骨髓样本的组织学特征。 2.维护全面的、前瞻性的、交互式的在线数据库，提供详细的临床和病理信息 核心接收到的样本的数据。 3.通过共享血液和骨髓促进AML-P01间和AML-P01外的协作 资源 细胞培养核心B部分的具体目标是为本计划项目提供细胞培养支持。 使用我们的组织培养试验，我们可以培养出早期和成熟的白血病以及正常的造血细胞。 祖先。此外，在与Core B的合作中，我们使用了荧光激活细胞分类器(FACS) 分离稀有的正常和白血病细胞群并研究不同抗白血病药物的作用 以及这些细胞的体外生长特性。这些检测方法非常适合于研究 在此PO1中调查的各种代理。

Effective tissue procurement and utilization is vital for meaningful translational research activities. The Core will work with each AML-P01 project and the Biostatistics and Data Management Core to ensure efficient and highly-coordinated procurement, use and storage of patient derived material. The Sample Distribution and Processing component, part A. Core has the following objectives: 1. Develop and maintain a repository of blood and bone marrow specimens, including intact cells, serum, cellular DMA, RNA and protein, from patients with a hematologic malignancy, including patients who are newly diagnosed, in remission and/or in relapse, receiving care or evaluation at M.D. Anderson Cancer Center. Distribute tissue specimens to AML-P01 investigators for analysis and provide expertise in the interpretation of studies performed on tissue sections within AML-P01 projects Provide comprehensive histological characterization of blood and marrow samples used in AML-P01 projects. 2. Maintain a comprehensive, prospective, interactive online database with detailed clinical and pathologic data for the samples received by the core. 3. Facilitate inter-AML-P01 and extra-AML-P01 collaborations through sharing of blood and marrow resources The specific aim of the cell culture core, Part B is to provide cell culture support for this Program Project. Using our tissue culture assays we can grow early and mature leukemic as well as normal hematopoietic progenitors. Furthermore, in collaboration with Core B, we use a fluorescence-activated cell sorter (FACS) to isolate rare populations of normal and leukemia cells and study the effect of various anti-leukemic agents and the in vitro growth characteristics of these cells. These assays are well suited to study the effects of various agents investigated in this PO1.