Mouse Core

Mouse Core的目的是提供一个集中的面向服务的设施， 每个参与项目的生产和维护转基因和基因破坏小鼠。鼠标核心 还将通过支持BUXCO系统的基础设施来服务于该计划的项目， 小鼠的侵入性抵抗力和顺应性测量，这将被大多数项目所利用。的 mouse core还将进行每个项目都将使用的气道解剖。正如所有项目建议的那样， 为了利用这些服务的组合，鼠标核心将是该计划项目的重要组成部分 格兰特.转基因/基因敲除生产单元由我们动物设施内的专用空间提供支持 装备齐全此外，Holtzman实验室常规使用BUXCO系统， 他的实验室里有一位精通这些技术的技术人员， 为本计划中的项目做这些实验的个人。这个核心是在我们的 肺科于1995年成立，已被项目研究者、合作研究者和 多年来，该应用程序的核心领导人（Holtzman，绿色，布罗迪，沃尔特，希普利）。它的工作人员是 在这一领域具有广泛专业知识和生产力的集体记录的人员。核心 负责产生超过100个单独的转基因和敲除品系，具有多个 创始人/嵌合体。许多已经和将要由这个核心制造的老鼠将进一步 有助于促进项目负责人之间的协作互动。目前，Mouse Core 部分由另一个计划项目赠款（罗伯特M。高级，PI）和SCOR赠款（迈克尔J。 Holtzman，PI）。该核心也被华盛顿大学的其他研究人员使用。因此，这将允许 我们可以实现规模经济和降低每只小鼠的生产成本。

The purpose of the Mouse Core will be to provide a centralized service-oriented facility to insure the capacity of each participating project to produce and maintain transgenic and gene-disrupted mice. The mouse core will also serve the projects of this Program by supporting the infrastructure of the BUXCO system for making invasive resistance and compliance measurements in mice, which will be utilized by most projects. The mouse core will also carry out airway dissections that will be utilized by each project. As all projects propose to utilize a combination of these services, the Mouse Core will be an important part of this Program Project Grant. The transgenic/knockout production unit is supported by dedicated space within our animal facility and is fully equipped. In addition, the Holtzman laboratory routinely utilizes the BUXCO system and carries out isolations of mouse airways, and a technician in his laboratory who is proficient in these techniques will be the individual doing these experiments for projects in this Program. This core was developed in our Pulmonary Division in 1995 and has been extensively utilized by Project Investigators, Co-Investigators, and Core Leaders on this application for many years (Holtzman, Green, Brody, Walter, Shipley). It is staffed by personnel with a collective extensive track record of expertise and productivity in this area. The core has been responsible for generating well over 100 separate transgenic and knockout lines, with multiple founders/chimeras of each. Many of the mice which have been and will be made by this core will further serve to facilitate collaborative interactions amongst Project Leaders. The Mouse Core is currently also supported in part by another Program Project Grant (Robert M. Senior, PI) and SCOR grant (Michael J. Holtzman, PI). The core is also utilized by other investigators at Washington University. Thus, this will allow us to realize an economy of scales and lower production costs per mouse generated.