Inflammatory Responses To Bronchial Endotoxin Instillati

给予人体内毒素可以提供一种独特的方法来评估感染期间发生的早期炎症反应。表征这些反应和控制它们的机制是重要的，因为这些炎症反应有助于脓毒性休克和器官衰竭的发展。根据方案92-CC-0141，将评价将内毒素直接滴入肺亚段的影响。将在内毒素滴注后6、24或48小时进行序贯支气管肺泡灌洗。分析将包括以下内容：急性期细胞因子的支气管肺泡灌洗;嗜中性粒细胞和淋巴细胞亚群的流式细胞术;以及全身和炎症效应，包括急性期细胞因子释放、骨髓细胞募集和急性期蛋白质释放的启动（Am J Respir Crit Care Med 2001;163：1591）。这些观察结果将有助于确定细菌内毒素引起的急性肺部炎症的发生和消退中的重要事件。迄今为止，已经使用这种局部内毒素滴注模型对63名受试者进行了研究。

Administration of endotoxin to humans allows a unique way to evaluate the early inflammatory reactions that occur during infection. Characterizing these responses and the mechanisms that control them is important because these inflammatory responses contribute to the development of septic shock and organ failure. Under protocol 92-CC-0141, the effects of direct instillation of endotoxin into lung subsegments will be evaluated. Sequential bronchoalveolar lavage will be performed at 6, 24, or 48 hours after endotoxin instillation. Analyses will include the following: bronchoalveolar lavage for acute phase cytokines; flow cytometry of neutrophils and lymphocyte subpopulations; and systemic and inflammatory effects including acute phase cytokine release, recruitment of cells from the marrow and the initiation of acute phase protein release (Am J Respir Crit Care Med 2001;163:1591). These observations will be useful in defining important events in the initiation and resolution of acute lung inflammation to bacterial endotoxin. To date, 63 subjects have been studied using this model of local endotoxin instillation.