Multi-channel High-Resolution and High-Throughput MS for Proteomics and Glycomics

描述(申请人提供)：尽管电喷雾电离(ESI)和基质辅助激光解吸/电离(MALDI)技术都已与高分辨率串联质谱仪(MS)对接，但目前只有MALDI串联MS(或MS/MS)适用于蛋白质组和糖组学的高通量应用。ESI-MS/MS的吞吐量通常受到通常与ESI-MS/MS仪器结合使用的慢速液相色谱(LC)技术的限制。这项建议的目的是开发一种新的高分辨率和高通量的ESI串联质谱仪，用于蛋白质组学和糖组学中的重要应用，包括增加蛋白质鉴定和结构鉴定的吞吐量，翻译后修饰的表征，以及区分蛋白质差异表达的相对定量。新的仪器设计基于高效和完全独立的多通道高分辨率串联MS，其中真空系统、数据和控制系统以及大部分电子学和质量分析仪在所有MS通道之间共享，通过将一个MS连接到多个LC系统，大大降低了整个MS系统的成本，并提高了整个MS系统的吞吐量。蛋白质组学和糖组学工具的应用在现代生命科学、药物开发和临床应用中发挥着重要作用。我们提出了一种新的技术来提高蛋白质鉴定和结构鉴定的吞吐量，这是当今蛋白质组学和糖组学中的一种基本策略。

DESCRIPTION (provided by applicant): Although both electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) techniques have been interfaced with high resolution tandem mass spectrometers (MS) only MALDI tandem MS (or MS/MS) is currently suitable for high throughput applications in proteomics and glycomics. The ESI-MS/MS throughput is usually limited by slow liquid chromatography (LC) technique usually utilized in conjunction with ESI-MS/MS instrumentation. The purpose of this proposal is to develop a new high resolution and high throughput ESI tandem mass spectrometer for important applications in proteomics and glycomics, including increasing throughput of protein identification and structure elucidation, characterization of post-translational modifications, and relative quantitation to distinguish differential expression of proteins. The new instrument design is based on efficient and fully independent multi-channel high resolution tandem MS in which vacuum system, data and control system, and substantial part of electronics and mass analyzer are shared between all MS channels, resulting in substantial cost reduction and increase of throughput of the total MS system by interfacing one MS to multiple LC systems. The application of proteomics and glycomics tools plays an important role in modern life sciences, drug discovery, and clinical applications. We propose a new technology for increasing throughput of protein identification and structure elucidation using mass spectrometry, an essential strategy in proteomics and glycomics today.