Instrument for Determining Intramolecular Interactions

描述（由申请人提供）： 这项工作的目标是发展质谱能力和技术，以确定二级和三级结构的生物分子。工作的初始部分将涉及通过在前端添加高场不对称波形离子迁移谱仪/四极杆质量过滤器来修改扇形质谱仪。在仪器的四极和扇形部分之间的界面中将是几个反应区域，其中低或高能量碰撞诱导解离（CID）和电子捕获诱导解离。研究的主要领域将涉及确定从CID获得二级（也许是三级）结构的能力。这些实验将涉及离子迁移率和H/D交换与CID的组合。这些实验将与凝聚相实验相补充，以试图确定溶液和气相中的结构之间是否存在相关性。

DESCRIPTION (provided by applicant): The goal of this work is-to develop mass spectrometry capabilities and techniques to determine the secondary and tertiary'structure of biomolecules. The initial part of the work will involve modifying a sector mass spectrometer by adding a high-field asymmetric waveform ion mobility spectrometer/quadrupole mass filter to the front end. In the interface between the quadrupole and sector portions of the instrument will be several reaction regions in which low or and high energy collision-induced dissociation (CID), and electron capture induced dissociation. The primary area of investigation will involve determining the ability to obtain secondary (and perhaps tertiary) structure from CID. These experiments will involve the combination of ion mobility and H/D exchange with CID. These experiments will be complemented with condensed phase experiments in an attempt to determine if there is a correlation between structures in solution and in the gas phase.