JEOL 2100 LAB6 TRANSMISSION ELECTRON MICROSCOPE WITH CAMERA: INFECTIOUS DISEASE

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：我们已经组建了一个由5名NIH资助的研究人员组成的主要用户组，其中有8个项目，以及一个由9名次要用户组成的额外用户组，其中大多数也是NIH资助的研究人员。我们的目标是用配备GATAN Ultrascan 1000数码相机的JEOL 2100 LaB 6型显微镜取代我们现有的使用了18年的Hitachi H600显微镜。该显微镜具有我们现有仪器所不具备的众多功能，其中包括数字图像采集、多样品保持器、电动5轴精密测角仪、200 kV发射电流和远程访问显微镜。高分辨率2K X 2K GATAN相机附带的软件包是行业标准，能够生成断层重建以及进行图像采集和分析。此外，如果这项申请得到资助，MCW管理部门将购买一台新的Boeckler/RMC超薄切片机和一台Leica EM Pact高压冷冻系统。所有这些功能将大大提高在MCW进行的EM工作的质量和及时性。MCW已经拥有一个共享的EM设施，该设施将为操作、维护和使用新设备提供基础设施和技术专业知识。这个设施是由私家侦探建立的。与其他主要用户之一Besharse博士合作，并由一位拥有25年以上经验的电子显微镜专家（克莱夫威尔斯先生）干练地指导。用户组包括微生物学和分子遗传学，细胞生物学，神经生物学和解剖学，生物化学和医学部门的研究人员。如果可用性允许，也欢迎其他调查人员使用该设施。有关仪器使用和用户费用的政策将由内部咨询委员会审查和讨论。新的仪器将为MCW提供最先进的设施，为NIH资助的广大教师提供培训和EM服务。主要用户组将要进行的工作将推进我们对牛痘病毒复制和形态发生的理解（Traktman，2个项目），Per 3基因在光感受器变性中的作用和光感受器中光转导蛋白的鞭毛内转运过程（Besharse，2个项目），胃肠发育和肝脏发育的转录控制（邓肯，2个项目），脊椎动物视网膜分层和青光眼基因相互作用（链接，2个项目），振动损伤和长期航天对人类骨骼肌的影响（莱利，2个项目）。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): We have assembled a major user group of 5 NIH-funded investigators with 8 projects, and an additional group of 9 minor users, most of whom are also NIH-funded investigators. Our goal is to replace our existing, ~18-year old Hitachi H600 microscope with a JEOL 2100 LaB6 version microscope equipped with a GATAN Ultrascan 1000 digital camera. This microscope has numerous capabilities that our existing instrumentation does not have, among which are digital image acquisition, multi-specimen holder, motorized 5-axis precision goniometer, 200 kV emission current, and remote access microscopy. The software package provided with the high resolution 2K X 2K GATAN camera is the industry standard, and has the capable of generating tomographic reconstructions as well as enabling image acquisition and analysis. In addition, if this application is funded, the MCW administration will purchase a new Boeckler/RMC Ultramicrotome and a Leica EM Pact high pressure freezing system. All of these features will vastly improve the quality and timeliness of the EM work that can be performed at MCW. MCW already has a shared EM facility which will provide the infrastructure and technical expertise for operating, maintaining and using the new equipment. This facility was established by the P.I. in cooperation with Dr. Besharse, one of the other major users, and is ably directed by an electron microscopist with more than 25 years of experience (Mr. Clive Wells). The user group comprises investigators in the Departments of Microbiology & Molecular Genetics, Cell Biology, Neurobiology & Anatomy, Biochemistry, and Medicine. Additional investigators will also be welcome to use the facility as availability permits. Policies regarding instrumentation access and user fees will be reviewed and discussed by an Internal Advisory committee. The new instrumentation will provide MCW with a state-of-the art facility which will provide training and EM services to a broad group of NIH- funded faculty. The work to be performed by the major user group will advance our understanding of the replication and morphogenesis of vaccinia virus (Traktman, 2 projects), the role of the Per3 gene in photoreceptor degeneration and the process of intraflagellar transport of phototransduction proteins in photoreceptors (Besharse, 2 projects), the transcriptional control of gastrointestinal development and hepatic development (Duncan, 2 projects), vertebrate retinal lamination and glaucoma gene interactions (Link, 2 projects), and vibration injury and effect of prolonged spaceflight on human skeletal muscle (Riley, 2 projects).