JEOL 2100 LAB6 TRANSMISSION ELECTRON MICROSCOPE WITH CAMERA: EYE & VISION

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：我们已经组建了一个由5名美国国立卫生研究院资助的研究者组成的主要用户组，有8个项目，还有一个由9名次要用户组成的额外用户组，其中大多数也是美国国立卫生研究院资助的研究者。我们的目标是用配备GATAN Ultrascan 1000数码相机的JEOL 2100 LaB6版显微镜取代现有的18年历史的日立H600显微镜。该显微镜具有许多我们现有仪器所不具备的功能，其中包括数字图像采集，多标本夹，电动五轴精密测角仪，200kv发射电流和远程访问显微镜。高分辨率2K X 2K GATAN相机提供的软件包是行业标准，能够生成层析重建以及实现图像采集和分析。此外，如果这项申请得到资助，MCW管理部门将购买一台新的Boeckler/RMC超微显微镜和一台徕卡EM Pact高压冷冻系统。所有这些功能将大大提高可在MCW执行的EM工作的质量和及时性。MCW已经拥有一个共享的EM设施，该设施将为新设备的操作、维护和使用提供基础设施和技术专长。该设施是由pi与其他主要用户之一Besharse博士合作建立的，并由具有25年以上经验的电子显微镜学家（Clive Wells先生）干干地指导。用户群包括微生物学与分子遗传学、细胞生物学、神经生物学与解剖学、生物化学和医学系的研究人员。如果条件允许，也欢迎其他调查人员使用该设施。有关仪器访问和用户费用的政策将由内部咨询委员会审查和讨论。新仪器将为MCW提供最先进的设施，为NIH资助的教师提供培训和EM服务。主要用户组将开展的工作将促进我们对牛痘病毒的复制和形态发生（Traktman， 2个项目），Per3基因在光感受器变性中的作用和光转导蛋白在光感受器中的束内运输过程（Besharse， 2个项目），胃肠道发育和肝脏发育的转录控制（Duncan， 2个项目），脊椎动物视网膜层压和青光眼基因相互作用（Link， 2个项目），以及长时间太空飞行对人体骨骼肌的振动损伤和影响（Riley， 2个项目）。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): We have assembled a major user group of 5 NIH-funded investigators with 8 projects, and an additional group of 9 minor users, most of whom are also NIH-funded investigators. Our goal is to replace our existing, ~18-year old Hitachi H600 microscope with a JEOL 2100 LaB6 version microscope equipped with a GATAN Ultrascan 1000 digital camera. This microscope has numerous capabilities that our existing instrumentation does not have, among which are digital image acquisition, multi-specimen holder, motorized 5-axis precision goniometer, 200 kV emission current, and remote access microscopy. The software package provided with the high resolution 2K X 2K GATAN camera is the industry standard, and has the capable of generating tomographic reconstructions as well as enabling image acquisition and analysis. In addition, if this application is funded, the MCW administration will purchase a new Boeckler/RMC Ultramicrotome and a Leica EM Pact high pressure freezing system. All of these features will vastly improve the quality and timeliness of the EM work that can be performed at MCW. MCW already has a shared EM facility which will provide the infrastructure and technical expertise for operating, maintaining and using the new equipment. This facility was established by the P.I. in cooperation with Dr. Besharse, one of the other major users, and is ably directed by an electron microscopist with more than 25 years of experience (Mr. Clive Wells). The user group comprises investigators in the Departments of Microbiology & Molecular Genetics, Cell Biology, Neurobiology & Anatomy, Biochemistry, and Medicine. Additional investigators will also be welcome to use the facility as availability permits. Policies regarding instrumentation access and user fees will be reviewed and discussed by an Internal Advisory committee. The new instrumentation will provide MCW with a state-of-the art facility which will provide training and EM services to a broad group of NIH- funded faculty. The work to be performed by the major user group will advance our understanding of the replication and morphogenesis of vaccinia virus (Traktman, 2 projects), the role of the Per3 gene in photoreceptor degeneration and the process of intraflagellar transport of phototransduction proteins in photoreceptors (Besharse, 2 projects), the transcriptional control of gastrointestinal development and hepatic development (Duncan, 2 projects), vertebrate retinal lamination and glaucoma gene interactions (Link, 2 projects), and vibration injury and effect of prolonged spaceflight on human skeletal muscle (Riley, 2 projects).