AB 3730XL, 96 Cap DNA Analyzer

描述（由申请人提供）：基因分型的需求正在迅速增加，通过大型关联研究对复杂性状的研究，大量的样本和SNP。与此同时，对微卫星基因分型和测序的需求至少在目前的水平上继续存在。因此，必须提高快速、廉价、高通量测序和基因分型的能力。最近实验室仪器的迅速发展能够很好地跟上这一日益增长的需求。然而，这种高科技伴随着高昂的价格。事实证明，除了规模最大、资金最充足的实验室之外，任何实验室都不可能找到资源，随着研究的进展，从一种尖端技术转移到另一种尖端技术。为了解决这个问题，加州大学洛杉矶分校承诺通过提供最先进的核心设施系统来支持和促进所有科学家的研究。这些核心的人员配备和设备，使科学家能够将更多的时间集中在解决智力问题上，而不是获取和学习新技术。UCLA测序和基因分型核心服务于当地科学界的大型用户群的研究需求。我们已经确定了一种廉价的方法来产生越来越多的SNP基因型的未来需要。为了满足这一需求，同时继续支持我们所服务的科学家的测序和微卫星需求，核心需要更新其能力。我们正在申请资金用于AB 3730XL，96毛细管DNA分析仪。核心已经成功地运行了五年多的旧型号的这项技术。目前有两台AB 3700毛细管仪器和一台AB 3730S，48毛细管仪器。这三种仪器目前正在接近能力，每天生成DNA序列，微卫星和SNP。3700台仪器已经连续运行了四年，很快就需要更换。3700不能运行SNP基因型，它产生的序列具有较短的读取长度和较低的质量。3700的效率较低，更容易出现故障，并且比3730的运行成本和每个样品的成本更高。该模式已停止使用，在申请供资期后两年内将不再得到支持。因此，有必要用要求的3730仪器更换3700。

DESCRIPTION (provided by applicant): The demand for genotyping is increasing rapidly, fuelled by research into complex traits through large association studies, with large numbers of samples and SNPs. At the same time, the need for microsatellite genotyping and sequencing continues to exist in at least their current levels. Therefore, the capacity for rapid, cheap, high-throughput sequencing and genotyping must increase. The recent rapid explosion in laboratory instrumentation is well able to keep pace with this increasing need. However, this high technology comes with a high price tag. It can prove impossible for any but the largest, most well-funded laboratories to find the resources to move from one cutting-edge technology to the next as their research progresses. To address this problem, the University of California, Los Angeles has made a commitment to supporting and facilitating the research of all its scientists by providing a system of state-of-the-art Core facilities. These Cores are staffed and equipped to allow scientists to concentrate more time on intellectual problem solving, and less on acquiring and learning new technologies. The UCLA Sequencing and Genotyping Core serves the research needs of a large user base in the local scientific community. We have identified a coming need for an inexpensive method to generate increasingly large numbers of SNP genotypes. In order to meet this need, while at the same time continuing to support the sequencing and microsatellite requirements of the scientists we serve, the Core needs to update its capacity. We are requesting funds for an AB 3730XL, 96 Capillary DNA Analyzer. The Core has been successfully operating older models of this technology for over five years. Currently there are two AB 3700 capillary instruments, and one AB 3730S, 48 capillary instrument. These three instruments are currently running close to capacity, generating DNA sequence, microsatellites, and SNPs on a daily basis. The 3700 instruments have been in continuous operation for four years, and will need to be replaced very soon. The 3700 cannot run SNP genotypes, and the sequence it produces has shorter read lengths and inferior quality to the 3730. The 3700 is less efficient, more prone to break-down, and has higher running costs and per-sample costs than the 3730. The model has been discontinued, and will not be supported within two years of the requested funding period. Therefore, it is necessary to replace the 3700s with the requested 3730 instrument.