7900HT FAST REAL-TIME PCR SYST: COOLEY'S ANEMIA, BETA-THALASSEMIA

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：本NCRR共享仪器提案寻求资金用于升级应用生物系统公司7900HT快速实时PCR系统，用于威尔医学院（WMC）的微阵列核心设施（MCF）。目前的实时PCR仪（Taqman 7700）安装于1997年，已被WMC的研究人员大量使用来验证他们的微阵列数据。然而，该系统存在严重的缺陷，包括工作站的不稳定性，导致频繁的崩溃，从而导致整个PCR反应的失败；它的运行时间也很长，这限制了每天的使用次数。这些缺陷阻碍了WMC的研究。升级后的7900HT系统具有更高的高性能，包括用户可互换块格式，快速PCR选项和384孔TaqMan低密度阵列。7900HT将成为WMC独特而宝贵的资源，作为一种实时定量PCR系统，它具有更广泛的功能，更高的吞吐量，比学院现有的所有实时PCR仪器更快，更简单，更准确的处理。这些改进对于基础和临床研究人员验证微阵列数据至关重要；它们对于WMC正在进行的高通量功能基因组学、突变和缺失研究也是必不可少的。这项要求升级到最先进的应用生物系统7900HT快速实时PCR系统将大大提高MCF服务的质量和数量，以支持美国国立卫生研究院在WMC资助的广泛项目。目前由NIH资助的30名WMC教师聚集在一起，加强MCF的这些服务，他们的研究项目的成功本质上取决于这些服务。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This NCRR shared instrumentation proposal seeks funding for upgrading an Applied Biosystems 7900HT Fast Real-Time PCR System for the Microarray Core Facility (MCF) of Weill Medical College (WMC). The current real-time PCR machine (Taqman 7700), installed in 1997, has been heavily used by WMC's investigators to validate their microarray data. However, this system has severe defects, including the instability of the workstation, which causes frequent crashes and thereby the failure of entire PCR reactions; it also has a long running time, which limits the number of uses per day. These defects are hampering research at WMC. The upgraded 7900HT system has an improved capability of high-performance, which includes user-interchangeable block formats, a Fast PCR option and a 384-well TaqMan Low Density Array. The 7900HT will be a unique and valuable resource at WMC as a real-time quantitative PCR system with a greater range of functions, higher throughput capacity, faster, simpler and more accurate processing than all current real-time PCR instruments in the college. These improvements are critical to the validation of microarray data for basic and clinical researchers; they are also essential for high throughput functional genomics, mutation and deletion studies that are being carried out at WMC. This requested upgrade to the state-of-the-art Applied Biosystems 7900HT Fast Real-Time PCR System will greatly enhance the quality and quantity of the services at MCF in support of a wide range of NIH-funded projects at WMC. Thirty WMC faculty members, who are currently supported by NIH funding, have come together to strengthen these services of MCF, on which the success of their research projects inherently depend.