CONFOCAL MICROSCOPE FOR CAMPUS EXPANSION IN THE CBH BUILDING: AGING

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：该提案要求资助购买共享使用的共聚焦激光扫描显微镜，蔡司LSM 510系统。该生物分析系统将为北德克萨斯大学健康科学中心（UNTHSC）越来越多的美国国立卫生研究院资助的神经科学研究人员提供服务，该中心最近搬到了新建的生物健康中心（CBH大楼），这是一个新的场外位置，是UNTHSC校园扩建的一部分。由于使用共聚焦显微镜进行研究的教师和学生数量的强劲增长，目前UNTHSC（位于RES大楼）的一台共聚焦显微镜的能力不足。此外，更重要的是，横跨校园的新地点不允许大多数nih资助的共聚焦显微镜研究人员有效地使用这项技术，因为他们的细胞和组织为基础的分析和模型系统不允许运输或暴露于与运输相关的条件下到远程共聚焦显微镜设施。目前，在CBH的UNTHSC的非现场位置没有共聚焦显微镜，也没有直接相邻的建筑物可以允许研究人员进行这种专门的数据采集。所要求的仪器将被安置在CBH504的显微镜核心设施中，该设施位于中心位置，允许所有用户不仅从CBH大楼内直接访问，而且允许毗邻各自实验室的所有主要用户直接访问。蔡司LSM 510系统是一种最先进的仪器，作为一种得到良好支持和建立的多用户仪器，具有良好的记录。它将满足当前和未来对先进成像的需求，并使UNTHSC的教学技术现代化。目前共聚焦显微镜在CBH领域的研究需要包括：1)测量活细胞中信号分子的亚细胞变化；2)以常规荧光显微镜无法达到的分辨率对新分离的细胞和组织进行光学切片；3)确定低丰度蛋白质随时间的分布，同时尽量减少对细胞生理的影响；4)阐明基因修饰细胞中蛋白表达和转运的机制；5)对新型神经保护剂进行体内外构效关系分析。目前的申请将主要服务于8个主要研究小组，这些小组由来自不同NIH研究所的同行评审拨款资助。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The proposal requests funding for the acquisition of a shared-use confocal laser-scanning microscope, the Zeiss LSM 510 system. This bioanalytical system will serve an increasing community of NIH-funded neuroscience researchers at the University of North Texas Health Science Center (UNTHSC) that have recently moved to the newly constructed Center for BioHealth (CBH building), a new off-site location that is part of a campus expansion of the UNTHSC. The capabilities of the one current confocal microscope at UNTHSC (located in the RES building) are insufficient due to the strong increase in the number of faculty and students using confocal microscopy for their research. In addition and more importantly, the new location across campus does not allow the majority of NIH-funded investigators of confocal microscopy to effectively use this technology, because their cell and tissue based assays and model systems do not tolerate transport or exposure to conditions associated with transport to a remotely located confocal microscopy facility. There is currently no confocal microscope at the off-site location at UNTHSC, in CBH, nor in a directly adjacent building that would allow researchers to perform this specialized data acquisition. The requested instrument will be housed in the microscopy core facility in CBH504, centrally located to allow all users direct access not only from within the CBH building but also for all major users adjacent to their respective laboratories. The Zeiss LSM 510 system, a state-of-the-art instrument, has a proven track record as a well-supported and established multi-user instrument. It will meet the current increased and future needs for advanced imaging, as well as modernize UNTHSC's teaching technology. Current research needs for a confocal microscope in CBH include: 1) measuring subcellular changes in signaling molecules in live cells; 2) optical sectioning of freshly isolated cells and tissue at a resolution that cannot be achieved with conventional fluorescence microscopy; 3) determining the distribution of low-abundance proteins over time while minimally affecting cell physiology; 4) elucidating mechanisms protein expression and trafficking in genetically modified cells; 5) conducting analyses of structure-activity relationships of novel neuroprotective agents in vitro and in vivo. The current application will primarily serve eight major groups around principal investigators that are extramurally funded by peer-reviewed grants from different NIH institutes.