OLYMPUS FV 1000 CONFOCAL MICROSCOPE FOR NEUROBIOLOGY AND CELLULAR BIOLOGY RESEAR

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：这份建议书申请资金购买奥林巴斯FluoView(FV)1000光谱单元激光扫描共聚焦显微镜，以供NIH资助的研究人员互动小组使用，该小组主要位于莱特州立大学的解剖和生理学系。该单元还将与分布在医学院和科学与数学学院其他几个系的其他调查人员共享使用。这个新的共焦系统将用于两个主要目的，1)更换我们已有6年历史的奥林巴斯FX和2)升级我们的共焦仪器能力。新系统带来的主要进步包括：a)多个荧光标记，超越了我们目前的两个通道限制；b)激光照明的最佳控制；c)改进的荧光分离，即使在发射曲线密切相关的情况下也是如此；d)改进了文件处理、保存和导出，以便在2D和3D空间中进行进一步的定量分析。该仪器将主要由一个由五名研究人员组成的核心小组使用，该小组将组成一个合作单位，在发育、损伤和损伤恢复期间修改突触和回路方面拥有共同的项目和共同的科学兴趣。他们还在成像设备和定量方法方面积累了广泛的专业知识，其中许多需要改进奥林巴斯FV 1000系统的能力。来自更多使用者的研究课题多种多样，从神经元的容量或pH控制到细胞钙转运机制和红细胞和神经元的氯离子动态平衡，再到中枢神经系统对呼吸功能的控制。大多数研究人员(主要或次要用户)都是通过NIH提供资金的。为了促进维护和公平使用，该仪器将整合到脑研究中心的显微镜和成像设施中。这一核心设施成立于2000年，任务是向主要(但不限于)医学院的研究人员提供成像和组织学需求(电子显微镜、图像分析、神经元追踪、共焦显微镜、显微切割术和超显微切割术)，重点是神经科学研究。因此，收购奥林巴斯FV 1000系统将大大增强核心研究人员的研究计划，但也将对莱特州立大学更广泛的研究社区产生有益的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This proposal requests funds to purchase an Olympus Fluoview (FV) 1000 Spectral Unit Laser Scanning Confocal Microscope to be utilized by an interactive group of NIH-funded investigators, primarily located in the Department of Anatomy and Physiology at Wright State University. The unit will be also available for shared use with other investigators distributed throughout several other Departments of the Medical School and the College of Science and Mathematics. This new confocal system will serve two major purposes, 1) replacement of our six year-old Olympus FX and 2) upgrade our confocal instrumentation capabilities. Major advances provided by the new system include a) multiple fluorochrome labeling beyond our current two channel limitations; b) optimal controls of laser illumination; c) improved fluorochromes separation, even when emission curves are closely related and d) improved file handling, save and export for further quantitative analyses in 2D and 3D space. The instrument will be mostly used by a core group of five investigators that will form a collaborative unit with shared projects and common scientific interests on the modification of synapses and circuits during development, injury and recovery from injury. They have also accumulated a broad range of expertise with imaging equipment and quantitative methods, many of which require the improved capabilities of the Olympus FV 1000 system. The research topics from further users are varied and range from volume or pH control in neurons to cellular calcium transport mechanisms and chloride homeostasis in red blood cells and neurons to central nervous system control of respiratory function. The majority of investigators (major or minor users) are funded through NIH. To promote maintenance and equitable use, the instrument will be integrated within the microscopy and imaging facilities of the Center for Brain Research. This core facility was established in 2000 with the mission of providing imaging and histological needs (electron microscopy, image analysis, neuron tracing, confocal microscopy, microtomy and ultramicrotomy) to researchers located primarily (but not exclusively) in the School of Medicine and with an emphasis in Neuroscience research. As such, the acquisition of the Olympus FV 1000 systems will significantly enhance the research programs of the core investigators, but it will also have a beneficial impact on the broader research community at Wright State University.