Arcturus Veritas Microdissection Microscope

描述（由申请人提供）：本申请要求Arcturus Veritas激光捕获显微切割（LCM）显微镜配备紫外切割激光器、荧光成像和高分辨率显微镜物镜的主要选项。我们的5个主要用户需要该仪器来执行先进的LCM程序，这超出了校园内现有的两台6年前的仪器的能力。具体而言，用户将受益于Veritas的以下能力：（1）使用紫外激光切割来补充经典的红外激光附着方案，（2）允许样品制备和样品收集方案的更多多样性，（3）在一个会话中自动收集多达3个载玻片的多种细胞类型，以及（4）提高选择和收集目标区域的速度，以从样本中获得更大量的高质量RNA和蛋白质。Veritas将被纳入现有的中央（实时显微镜核心）设施，为辛辛那提大学和邻近的儿童医院研究基金会的研究人员提供服务。该机构靠近主要研究者的实验室，是所有研究者的中心位置，便于主要研究者每日监测设备状态。该申请得到了大学的大量兴趣，需求和财政支持的支持，并将为NIH资助的科学界提供强有力的服务。

DESCRIPTION (provided by applicant): This application requests an Arcturus Veritas laser capture microdissection (LCM) microscope outfited with the major options of an ultraviolet cutting laser, fluorescence imaging, and a high resolution microscope objective. Our 5 major users need the instrument to perform advanced LCM procedures that are beyond the capabilities of the two existing 6-year old instruments on campus. Specifically, users will benefit from the ability of the Veritas to (1) use UV laser cutting to supplement the classic IR laser attachment scheme, (2) allow more diversity in sample preparation and sample collection schemes, (3) automate collection of multiple categories of cell-type across up to 3 slides in one session, and (4) increase speed of selecting and collecting target regions to derive greater amounts of high quality RNA and protein from specimens. The Veritas will be incorporated into an existing centralized (Live Microscopy Core) facility that serves investigators at University of Cincinnati and the adjacent Children's Hospital Research Foundation. The facility is near the laboratory of the PI, a location that is central to all investigators and would facilitate daily monitoring of equipment status by the PI. The application is supported by substantial interest, need, and financial support at the University, and will serve a strongly NIH-funded scientific community.