BRUKER 800 MHZ TCI CRYOPROBE: STRUCTURE/FUNCTION OF HIV-1 VPR

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：为支持加州大学洛杉矶分校NIH、NSF和能源部资助的一组科学家的研究，申请800 MHz核磁共振低温探测仪，该探测仪将用于帮助解决主要用户建议研究的蛋白质、核酸、DNA-蛋白质、RNA-蛋白质和蛋白质-蛋白质复合体的结构和动力学。(1)蛋白质及其复合体的核磁共振研究(Clubb)，(2)核酸及其复合体的结构研究(Feigon)，(3)SAM结构域的结构与功能(Bowie)，(4)HIV-1 VPR和HHR23A相关细胞蛋白的结构/功能(Chen)，(5)XIs和Fis(Johnson)对噬菌体Lambda位点特异性DMA重组的调控，(6)生物分子核磁共振(LIN)中的混沌控制和敏感性增强(LIN)。探测器将位于加州大学洛杉矶分校-能源部核磁共振技术中心。它将显着提高该中心单台800兆赫光谱仪的灵敏度，使更多的项目能够受益于使用这台高场仪器。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Funding is requested for an 800 MHz NMR CryoProbe to support the research of a group of NIH, NSF and DOE funded scientists at UCLA, this probe will be used to help solve the structures and dynamics of proteins, nucleic acids, DNA-protein, RNA-protein, and protein-protein complexes proposed for investigation by the major users. The research projects of the six major users are described with the following titles: (1) NMR studies of proteins and their complexes (Clubb), (2) Structural studies of nucleic acids and complexes (Feigon), (3) SAM domain structure and function (Bowie), (4) Structure/function of HIV-1 Vpr and HHR23A associated cellular proteins (Chen), (5) Regulation of phage lambda site-specific DMA recombination by Xis and Fis (Johnson), and (6) Chaos Control and Sensitivity Enhancement in Biomolecular NMR (Lin). The probe will be located in the UCLA-DOE NMR Technology Center. It will significantly improve the sensitivity of the Center's single 800 MHz spectrometer enabling a greater number of projects to benefit from access to this high-field instrument.