ARCTURUS VERITAS MICRODISSECTION MICROSCOPE: PHYSIOLOGY

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：本申请需要一个Arcturus Veritas激光捕获显微解剖（LCM）显微镜，配备紫外切割激光，荧光成像和高分辨率显微镜物镜的主要选项。我们的5个主要用户需要该仪器执行先进的LCM程序，这超出了校园中现有的两台已有6年历史的仪器的能力。具体来说，用户将受益于Veritas的能力：(1)使用紫外激光切割来补充经典的红外激光附着方案，(2)允许更多的样品制备和样品收集方案的多样性，(3)在一次会议中自动收集多达3个载玻片上的多种类型的细胞类型，以及(4)提高选择和收集目标区域的速度，从而从标本中获得更多的高质量RNA和蛋白质。Veritas将被整合到现有的集中式（Live Microscopy Core）设备中，该设备为辛辛那提大学和邻近的儿童医院研究基金会的研究人员提供服务。该设施靠近监控方实验室，这是所有调查人员的中心地点，将有助于监控方每天监测设备状况。该申请得到了大学的大量兴趣、需求和财政支持，并将为nih资助的科学界提供强有力的服务。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This application requests an Arcturus Veritas laser capture microdissection (LCM) microscope outfited with the major options of an ultraviolet cutting laser, fluorescence imaging, and a high resolution microscope objective. Our 5 major users need the instrument to perform advanced LCM procedures that are beyond the capabilities of the two existing 6-year old instruments on campus. Specifically, users will benefit from the ability of the Veritas to (1) use UV laser cutting to supplement the classic IR laser attachment scheme, (2) allow more diversity in sample preparation and sample collection schemes, (3) automate collection of multiple categories of cell-type across up to 3 slides in one session, and (4) increase speed of selecting and collecting target regions to derive greater amounts of high quality RNA and protein from specimens. The Veritas will be incorporated into an existing centralized (Live Microscopy Core) facility that serves investigators at University of Cincinnati and the adjacent Children's Hospital Research Foundation. The facility is near the laboratory of the PI, a location that is central to all investigators and would facilitate daily monitoring of equipment status by the PI. The application is supported by substantial interest, need, and financial support at the University, and will serve a strongly NIH-funded scientific community.