ARCTURUS VERITAS MICRODISSECTION MICROSCOPE: PULMONARY DISEASES

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：本申请要求一台Arcturus Veritas激光捕获显微解剖(LCM)显微镜，配备紫外线切割激光、荧光成像和高分辨率显微镜物镜等主要选项。我们的5个主要用户需要该仪器来执行高级的LCM程序，这超出了校园中现有的两个6年前的仪器的能力。具体地说，用户将受益于VERITAS的能力：(1)使用UV激光切割来补充经典的红外激光附着方案，(2)允许样品准备和样品收集方案的更多多样性，(3)在一次会议中跨多达3张幻灯片自动收集多种类型的细胞类型，以及(4)提高选择和收集目标区域的速度，以从样品中提取更多的高质量RNA和蛋白质。Veritas将并入现有的集中式(实时显微镜核心)设施，为辛辛那提大学和邻近的儿童医院研究基金会的研究人员提供服务。该设施靠近PI的实验室，这是所有调查人员的中心位置，将有助于PI对设备状态的日常监测。这项申请得到了该大学巨大的兴趣、需求和财政支持，并将服务于NIH资助的科学界。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This application requests an Arcturus Veritas laser capture microdissection (LCM) microscope outfited with the major options of an ultraviolet cutting laser, fluorescence imaging, and a high resolution microscope objective. Our 5 major users need the instrument to perform advanced LCM procedures that are beyond the capabilities of the two existing 6-year old instruments on campus. Specifically, users will benefit from the ability of the Veritas to (1) use UV laser cutting to supplement the classic IR laser attachment scheme, (2) allow more diversity in sample preparation and sample collection schemes, (3) automate collection of multiple categories of cell-type across up to 3 slides in one session, and (4) increase speed of selecting and collecting target regions to derive greater amounts of high quality RNA and protein from specimens. The Veritas will be incorporated into an existing centralized (Live Microscopy Core) facility that serves investigators at University of Cincinnati and the adjacent Children's Hospital Research Foundation. The facility is near the laboratory of the PI, a location that is central to all investigators and would facilitate daily monitoring of equipment status by the PI. The application is supported by substantial interest, need, and financial support at the University, and will serve a strongly NIH-funded scientific community.