Biacore T100

描述（由申请人提供）：本提案中要求的仪器Biacore T100是一种测量表面等离子体共振（SPR）的自动化设备。Biacore仪器使用微流体系统测量SPR，该系统提供定量分析功能，可以详细研究两个分子之间结合的特异性、特定分子的浓度、蛋白质动力学和两种蛋白质之间的结合亲和力。实时定量数据的生成将帮助乔治城大学的研究人员进一步了解蛋白质、多肽和核酸相互作用的方式和原因。这些知识将用于推进基础和应用科学知识，重点关注癌症生物学和新的癌症治疗方法。Lombardi能够从NIH盈余中获得一个使用过的Biacore 1000，并生成初步数据，因为我们开始努力实现我们使用SPR的研究目标。与乔治城大学的B1000合作使我们有机会获得仪器和SPR的经验和专业知识。三个主要的用户，博士。Toretsky、Wellstein和Roy使用B1000为他们的项目生成关键数据。三位研究者都需要SPR的测量来推进他们的项目，现在他们正在用这些新数据准备论文。所有被列为主要用户的研究人员都资助了需要增强SPR功能的项目。琼斯博士提出了一个建议，其中包括一个需要SPR的目标。Drs。Pestell和Rui都有多个已建立的项目将从SPR中受益匪浅。下面的项目部分概述了这些好处。我们的科研需求已经超过了B1000的容量。这些需求包括增强检测小分子相互作用的能力、温度动力学和更快的吞吐量，以适应乔治敦Lombardi癌症中心日益增长的兴趣和增加的用户数量。T100的这些增强功能将使研究多种生物过程的项目得以推进。

DESCRIPTION (provided by applicant): The instrument requested in this proposal, the Biacore T100, is an automated device to measure surface plasmon resonance (SPR). The Biacore instrument measures SPR using a microfluidic system that provides quantitative analysis capabilities to study, in detail, the specificity of the binding between two molecules, concentrations of specific molecules, protein kinetics, and binding affinity between two proteins. The generation of real-time quantitative data will help Georgetown University investigators further understand how and why proteins, peptides, and nucleic acids interact. This knowledge will be used to advance both basic and applied scientific knowledge with a significant focus on cancer biology and novel cancer therapies. Lombardi was able to obtain a used Biacore 1000 from NIH surplus and generate preliminary data as we begin to work towards achieving our research goals using SPR. Working with the B1000 at Georgetown University has given us an opportunity to gain experience and expertise with the instrument and SPR. Three of the major users, Drs. Toretsky, Wellstein, and Roy, have used the B1000 to generate key data for their projects. All three investigators required the SPR measurements to advance their projects and are now preparing manuscripts with this new data. All investigators listed as Major Users have funded projects that require enhanced SPR capabilities. Dr. Jones has a proposal and includes an aim that requires SPR. Drs. Pestell and Rui both have multiple established projects that will benefit considerably from SPR. These benefits are outlined in their project sections below. Our scientific needs have exceeded the capacity of the B1000. These needs include the enhanced ability of detecting smaller molecule interactions, temperature kinetics, and more rapid throughput to accommodate the growing interest and increased number of users at Georgetown's Lombardi Cancer Center. These enhanced capabilities of the T100 will allow projects investigating many types of biologic processes to advance.