Performance of Rapid Tests for Detection of Avian Influe
Performance of Rapid Tests for Detection of Avian Influe
批准号:
7332561
负责人:
DANIEL P. FEDORKO
金额:
$0.0万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
中文摘要
我们的初步调查显示,两种快速抗原捕获酶免疫测定法(EIA)在50,000 TCID50浓度下未检测出野生型H5N1型禽流感病毒[A/Vietnam/1203/2004(H5N1)]。我们用10倍稀释的野生型病毒系列(10 ~ 100万TCID50)对快速环境影响评价进行了挑战,发现环境影响评价在100万和10万TCID50下分别可以检测到a/ Vietnam/1203/2004(H5N1)和a/ Indonesia/05/2005(H5N1),但在任何稀释度下都不能检测到a/ Hong Kong/491/1997(H5N1)。这些数据表明,这些检测需要很高的病毒浓度,例如在感染早期有症状患者的鼻咽冲洗中发现的病毒浓度,以检测H5N1型禽流感病毒。
英文摘要
Our initial investigation revealed that two rapid antigen capture enzyme immunoassays (EIA) did not detect wild type avian influenza virus type H5N1 [A/Vietnam/1203/2004(H5N1)] at a concentration of 50,000 TCID50. We challenged the rapid EIAs with a 10 fold dilution series of wild type viruses from 10 to 1,000,000 TCID50 and found the EIAs could detect A/Vietnam/1203/2004(H5N1) and A/Indonesia/05/2005(H5N1) at 1,000,000 and 100,000 TCID50 respectively, but neither assay detected A/Hong Kong/491/1997(H5N1) at any dilution. These data suggest that these assays require high virus concentrations, such as those found in nasopharyngeal washes from symptomatic patients early in their infections, to detect avian influenza virus type H5N1.
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SURVIVAL OF MICROSPORIDIA AFTER EXPOSURE TO DISINFECTANTS AND ENVIRONMENTAL CONDI
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依托单位:
IN VITRO CULTURE AND SUSCEPTIBILITY TESTING OF MICROSPORIDIA
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批准号:6289459
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Performance of Rapid Tests for Detection of Avian Influe
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批准号:7215850
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:DANIEL P. FEDORKO
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依托单位:
国内基金
海外基金
Research on the Rapid Growth Mechanism of KDP Crystal
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批准号:10774081
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项目类别:面上项目
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资助金额:45.0万元
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批准年份:2007
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负责人:滕冰
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依托单位: