PRIMATE EMBRYO GENE EXPRESSION RESOURCE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。维护和扩展灵长类胚胎基因表达资源（PREGER），这是一个基于cDNA的资源，用于恒河猴卵母细胞和植入前胚胎的基因表达分析，并利用PREGER评估发育调控基因，以更好地了解灵长类胚胎发生的遗传调控。 我们提供了额外的样本来扩增PREGER，包括：1）在α鹅膏蕈碱中培养的2细胞期胚胎，以及来自FSH/hCG处理猴的囊胚期胚胎的内细胞团和滋养外胚层细胞，2）来自FSH处理猴的原核、2细胞、8细胞和囊胚期胚胎，以及3）来自未刺激猴的8细胞、桑椹胚和囊胚。在本报告所述期间，该项目将在西北炼油公司完成的工作已经完成。这项研究使用WNPRC动物服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To maintain and expand the Primate Embryo Gene Expression Resource (PREGER), which is a cDNA-based resource for gene expression analysis in rhesus monkey oocytes and preimplantation embryos, and to utilize the PREGER to evaluate developmentally regulated genes to better understand the genetic regulation of primate embryogenesis. We have provided additional samples to expand the PREGER including, 1) 2-cell stage embryos cultured in alpha amanitin, and inner cell mass and trophectoderm cells from blastocyst stage embryos obtained from FSH/hCG treated monkeys, 2) Pronucleate, 2-cell, 8-cell and blastocyst stage embryos from FSH treated monkeys, and 3) 8- cell, morula and blastocysts from nonstimulated monkeys. The work to be done at the WNPRC for this project was completed during the current report period. This research used WNPRC Animal Services.