THE PRIMATE EMBRYO GENE EXPRESSION RESOURCE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。目的：利用灵长类动物胚胎基因表达资源，以恒河猴为主要模式生物，对灵长类动物卵母细胞和着床前胚胎的基因表达进行快速、低成本的研究。PREGER包含超过160个信息丰富的样本，代表gv期卵母细胞、MIIstage期卵母细胞和从1细胞到孵化囊胚期的胚胎，通过体外或体内成熟、受精和培养的不同组合获得。这个广泛而有价值的样本集允许分析与理解基本灵长类胚胎学相关的基因表达，以及使用灵长类动物模型的临床程序对胚胎质量的潜在影响。PREGER中用于表达分析的Dot blots， cDNA文库，探针等工具可以分发给其他科学家，为灵长类胚胎的分子研究提供了前所未有的机会。图书馆可以用于无数其他目的，例如图书馆筛选和图书馆减法。我们建议保留这些资源，继续向科学界分发这些材料和分子工具，维护PREGER网站，将资源扩展到狒狒样本，以便评估灵长类物种之间观察的普遍性，并纳入人类和恒河猴胚胎干细胞系和恒河猴内细胞群细胞样本，以评估人类胚胎干细胞系的发育潜力。我们还建议应用一种新的抑制减法杂交方法，我们已经开发出与我们的扩增文库兼容的方法，以确定与早期灵长类胚胎发生相关的新的发育调节基因，这将有助于改进辅助生殖方法。这将促进基础研究，并可能支持新的临床方法，例如胚胎质量评估。所提出的活动将立即维护和扩大资源，产生关于灵长类胚胎的新的和基本的基本信息，并产生可能在临床实践中有用的新的分子方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: The Primate Embryo Gene Expression Resource permits the rapid and cost-effective study of gene expression in primate oocytes and preimplantation embryos using the rhesus monkey as the primary model organism. PREGER contains over 160 informative samples representing GV-stage oocytes, MIIstage oocytes, and embryos at the 1-cell through hatched blastocyst stages, obtained using different combinations of in vitro or in vivo maturation, fertilization, and culture. This extensive and valuable sample set permits analyses of gene expression related to understanding basic primate embryology, as well as potential effects of clinical procedures on embryo quality using a primate model. Dot blots for expression analysis, cDNA libraries, probes, and other tools in PREGER can be distributed to other scientists, providing unprecedented opportunities for molecular studies in primate embryos. The libraries can be employed for a myriad of other purposes, such as library screening and library subtraction. We propose to maintain the resource, continue to distribute these materials and molecular tools to the scientific community, maintain the PREGER website, extend the resource to encompass samples from baboon in order that the generality of observations among primate species may be evaluated, and incorporate samples of human and rhesus ES lines and rhesus inner cell mass cells for evaluating the developmental potency of human ES lines. We also propose to apply a novel method for suppression subtraction hybridization that we have developed to be compatible with our amplified libraries, in order to identify novel developmentally regulated genes that are relevant to early primate embryogenesis and that will assist improvements in assisted reproduction methods. This will facilitate basic research, and may support novel clinical approaches, e.g., in the evaluation of embryo quality. The proposed activities will at once maintain and expand the resource, produce new and fundamental basic information about primate embryos, and produce novel molecular approaches that may be useful in clinical practice.