TESTING HBV-KOREA SINGLE VIAL FORMULATION FOR IMMUNOGENICITY RESPONSE IN BABOONS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。这是一项由三部分组成的研究。第一部分的主要目的是从6只狒狒中收集两个淋巴结和一个皮肤活检组织，并在体外培养淋巴结细胞（6小时），以使用PCR分析测试基因产物的背景水平。本研究第2部分的目的是确定狒狒皮下注射1018 ISS、1018 ISS-PMXB、C274和C792制剂后，体外测定的免疫活性（第1部分）与体内活性之间是否存在相关性。对于研究的这一部分，将添加10只狒狒。第三部分的目的是测试狒狒对寡核苷酸的体外和体内反应。本实验将确定体外试验是否可用于检测用寡核苷酸/PMXB培养细胞后细胞活化的基因产物。这些产品的测试有可能帮助开发人类哮喘的治疗方法。这项研究目前正在进行中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is a three-part study. The main objective of part one is to collect two lymph nodes and one skin biopsy from 6 baboons, and culture lymph node cells in vitro (for 6 hours) to test for background levels of gene products, using PCR analysis. The purpose of part two of this study is to determine if there is a correlate between the immune activity measured in vitro (Part 1) with in vivo activity in baboons following subcutaneous injection with 1018 ISS, 1018 ISS-PMXB, C274 and C792 formulations. For this part of the study 10 baboons will be added. The purpose of part three is to test the in vitro and in vivo responses of baboons to oligonucleotides. This experiment will determine if in vitro assays are possible for the detection of gene products of cellular activation after culture of cells with oligonucleotides/PMXB. The testing of these products has the potential to aid in the development of a treatment for asthma in humans. This study is currently in progress.