MOTILITY OF LIVE CELLS QUANTIFIED BY FOURIER PHASE MICROSCOPY

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。利用傅里叶相显微镜从具有高横向分辨率和低噪声的透明结构中检索二维相移信息的能力，上皮癌细胞的运动已经被量化。用均方位移表示的结果表明，在细胞生命周期的各个阶段，细胞运动的性质是超扩散的。然而，有丝分裂细胞的有效扩散系数大约是非有丝分裂细胞的两倍。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Using the ability of Fourier phase microscopy to retrieve two-dimensional phase shift information from transparent structures with high transverse resolution and low-noise, motility of epithelial cancer cells has been quantified. The results in terms of the mean squared displacements suggest that the nature of cell motion is superdiffusive for cells at various stages of their life cycle. However, the effective diffusion coefficient for mitotic cells is approximately double that of non-mitotic cells.