HILBERT PHASE MICROSCOPY FOR INVESTIGATION OF RAPID DYNAMICS IN BIOLOGICAL SYST

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We developed Hilbert phase microscopy (HPM) as a novel optical technique for measuring high transverse resolution quantitative phase images associated with optically transparent objects. Due to its single-shot nature, HPM is suitable for investigating rapid phenomena that take place in transparent structures such as biological cells. The potential of this technique for studying biological systems was demonstrated with measurements on red blood cells, while its ability to quantify dynamic processes on the millisecond scale was exemplified with measurements on evaporating micron-size water droplets.