SPECTRAL ANALYSIS FLOW CYTOMETRY

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。一种具有光谱分析能力的流式细胞仪正在开发中。目前，所有的流式细胞仪都通过二向色镜和光谱滤光片分离荧光发射。这一发展的目标是通过用线阵CCD阵列记录光谱分散的光来分析荧光发射。初步计算表明，背面变薄的ccd阵列具有足够的灵敏度，可以探测来自激光光束中100微秒的单元的光谱分散光。从滨松株式会社获得了背面变薄的探测器评估试剂盒。它正被集成到现有的流式细胞仪中进行评估。在测试结果出来之前，我们将购买S电荷耦合器件阵列，永久集成到流量系统中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A spectral analysis capable flow cytometer is being developed. Currently, all flow cytometers separate fluorescence emissions by dichroic mirrors plus spectral filters. The goal of this development is to analyze the fluorescence emissions by recording with a line CCD array spectrally dispersed light. Preliminary calculations indicate that back thinned CCD arrays have sufficient sensitivity to detect spectrally dispersed light from cells that are in the laser beam for 100 microseconds. A back thinned detector evaluation kit has been obtained from Hamamatsu Corporation. It is being integrated into an existing flow cytometer for evaluation. Pending the outcome of the tests, we will purchase s CCD array for permanent integration into the flow system.