Facilitated assembly of RNA pol III RNPs - biogenesis of the human SRP complex

所有细胞的功能都需要利用和控制个体活动，通常是在大型分子机器内。例子包括复制DNA，合成蛋白质和产生能量（ATP）的机器。这些复合物必须以受控的方式正确组装，远离它们发挥功能的地方，因为部分或不正确组装的复合物可能会干扰正常过程。我们希望研究这样一种细胞机器的组装，即与细胞蛋白质分泌密切相关的信号识别颗粒。先前的理解表明，这发生在细胞核内，并且主要发生在称为核仁的亚核区域内，核仁也是许多其他复合物的组装场所，特别是蛋白质合成核糖体。我们的目标是确定组件的组装和定位路径的信号识别颗粒，并确定每个这些在这些事件中发挥的作用。

The function of all cells requires that individual activities are harnessed and controlled, often within large molecular machines. Examples include the machineries that replicate DNA, synthesize proteins and generate energy (ATP). These complexes must be assembled correctly in a controlled way, far from where they function, as partially or incorrectly assembled complexes might interfere with the normal processes. We wish to study the assembly of one such cellular machine, the signal recognition particle that is intimately associated with protein secretion from cells. Previous understanding indicates that this takes place within the nucleus, and largely within a subnuclear region termed the nucleolus, which is also the site of assembly of a number of other complexes, notably the protein synthesizing ribosome. Our aim is to identify components of the assembly and localisation path of signal recognition particle and to determine the role that each of these plays in these events.