DEVELOPING A PUBLIC CRYOEM REPOSITORY DATABASE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。在过去的十年中，使用电子冷冻显微镜确定的结构数量显著增长；预计这一趋势还会加速。为了提供一个合适的接口来描述和归档冷冻电镜结构，我们与PDB和EBI合作开发了一个冷冻电镜本体，不仅描述了结构本身，还描述了实验条件和相关的元数据项。我们希望建立一个社区共识的数据项目需要沉积三维密度图和原子模型衍生自低温电镜研究。此外，我们专注于改进目前的低温电子显微镜数据收集和沉积方法，以便实验人员将有一个通用的，易于使用的，一站式的低温电子显微镜结构沉积门户。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The past decade has seen a remarkable growth in the number of structures determined using electron cryomicroscopy; this trend is expected to accelerate. In order to provide a proper interface for describing and archiving cryoEM structures, we work with PDB and EBI in developing a cryoEM ontology to describe not only the structure itself, but also experimental conditions and pertinent meta-data items. We look to establishing a community consensus on the data items needed for deposition of 3D density maps and atomic models derived from cryoEM studies. Additionally, we are focused on improving the current cryoEM data collection and deposition methods so that the experimentalists will have a common, easy to use, one-stop portal for cryoEM structure deposition.