GROEL VARIANT

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。以前的研究无法捕获和研究GroEL- groes复合体的预触发状态并不奇怪，因为野生型GroEL只是短暂地（100毫秒左右）填充这种变构状态，对准备操作的时间太短了。相比之下，我们修改的GroEL变体似乎以相当慢的速度完成了这个转换。我们建议对这种新的GroEL变体进行高分辨率的观察，利用NCMI在通过冷冻电镜检查GroEL等分子方面取得的技术进步，可能会提供前所未有的GroEL- groes机器临界变构状态的快照，而我们目前对GroEL- groes机器知之甚少。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The inability of previous studies to trap and study a pre-triggered state of the GroEL-GroES complex is not surprising, given that wild-type GroEL populates this allosteric state only transiently (for a hundred milisec or so), much to short a time for ready manipulation. By contrast, our modified GroEL variant appears to move through this transition at a considerably slower rate. We propose that a high-resolution look at this new GroEL variant, exploiting the technical advances the NCMI has made in examining molecules like GroEL by cryo-EM, is likely to provide an unprecedented snap-shot of a critical allosteric state of the GroEL-GroES machine about which we currently know very little.