LIGANDS OF MOONLIGHTING RIBOSOME ASSEMBLY FACTORS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我们已经确定了存在于66S前核糖体中的几种基本的、保守的蛋白质，它们是核糖体组装所必需的。为了确定这些蛋白质在组装核糖体时的邻居或探索兼职伙伴，我们已经开始在P.James文库中筛选每种蛋白质的伙伴。我们还希望与YRC合作，使用这些蛋白质中的每一个作为诱饵，筛选您订购的双杂交文库。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have identified several essential, conserved proteins that are present in 66S pre-ribosomes and necessary for ribosome assembly. In order to identify neighbors of these proteins in assembling ribosomes or to explore for moonlighting partners, we have begun screening the P. James library for partners of each protein. We would also like to collaborate with the YRC to screen your ordered two-hybrid library, using each of these proteins as bait.