SALIVARY PROTEINS ASSOCIATED W/ ORAL ANTI FUNGICIDAL ACTIVITY

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。腮腺分泌的蛋白质是唾液的重要成分。这些蛋白质具有多种已知功能，包括参与消化和防止细菌和真菌生长。这些研究检查了人类唾液中具有抗真菌活性的成分。通过HPLC、亲和和离子交换色谱法部分纯化蛋白质。显示出抗真菌活性的级分用于蛋白质和质谱分析。使用MALDI-TOF和三重四极杆MS，已经鉴定了包括组蛋白和雌甾酮的几种蛋白质具有抗真菌活性。同样明显的是，与单个蛋白质相比，唾液蛋白质复合物表现出更高的抗真菌活性。正在使用蛋白质组学技术（包括凝胶内蛋白酶消化和串联MS）鉴定该活性复合物的蛋白质组分并进行结构表征。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Proteins secreted by the parotid glands are significant components of saliva. These proteins have a variety of known functions including involvement in digestion and prevention of bacterial and fungicidal growth. These studies examine the components of human saliva which have anti-fungicidal activity. Proteins are partially purified by HPLC, affinity and ion exchange chromatography. Fractions demonstrating anti-fungicidal activity are used for protein and mass spectral analysis. Using MALDI-TOF and triple quadrupole MS, several proteins including the histatins and statherin have been identified as having anti-fungicidal activity. It is also apparent that a salivary protein complex, as compared to individual proteins, demonstrates higher anti-fungicidal activity. The protein components of this active complex are being identified and structurally characterized using proteomic techniques, including in-gel protease digestion and tandem MS.