MASS SPECTROMETRY OF ANTIGENIC LIPOPEPTIDES: HIV

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。从一家商业公司获得了一种脂肽的合成版本，它是最早出现在艾滋病毒感染细胞中的化合物之一，并使用来自艾滋病毒患者捐赠者的细胞来生成T细胞系。进一步分析发现，合成的靶向脂肽不是纯的，而是一种混合物，而且靶向脂肽不是抗原性的，而是发现了两个新的抗原性成分。初步的质谱学研究表明，未知的抗原组分在结构上彼此非常相似，并且具有与预期合成结构相似的部分结构。BUSM MS资源实验室的质谱学研究包括使用MALDI和ESI Q-o-TOF和MALDI和ESI-FTMS仪器，以及使用酶消化已知和未知的化合物。正在进行的数据分析旨在确定这两种未知抗原化合物之间的结构差异，并阐明它们的完整结构。含有部分结构的化合物正在合成中，并进行活性测试。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A synthetic version of a lipopeptide that is one of the first compounds to appear in HIV-infected cells was obtained from a commercial company and employed to generate a T cell line, using cells from an HIV patient donor. On further analysis it was found that the synthetically produced version of the targeted lipopeptide was not pure, but in fact a mixture, and further, the targeted lipopeptide was not antigenic, but instead two novel components were found to be antigenic. Preliminary mass spectrometric investigations indicated that the unknown antigenic components are very similar in structure to each other and have a partial structure similar to the intended synthetic structure. Mass spectrometric studies at the BUSM MS Resource laboratory have included the use of MALDI and ESI Q-o-TOF and MALDI and ESI-FTMS instrumentation, as well as the use of enzymatic digestion of the known and unknown compounds. On-going data analysis is aimed at determining the structural difference between the two unknown antigenic compounds and elucidating their complete structures. Compounds that incorporate partial structures are being synthesized and tested for activity.