MASS SPECTROMETRY OF ANTIGENIC LIPOPEPTIDES: HIV

抗原性脂肽的质谱分析：HIV

• 批准号: 7602001
• 负责人: DAVID MOODY
• 金额: $ 2.51万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-03 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602001
• 关键词: Cell Line; Cells; Computer Retrieval of Information on Scientific Projects Database; Digestion; Electrospray Ionization; Funding; Grant; HIV; Institution; Investigation; Laboratories; Manuscripts; Mass Spectrum Analysis; Patients; Publications; Research; Research Personnel; Resources; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Structure; T-Lymphocyte; United States National Institutes of Health; analog; instrumentation; novel; posters

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A synthetic version of a lipopeptide that is one of the first compounds to appear in HIV-infected cells was obtained from a commercial company and employed to generate a T cell line, using cells from an HIV patient donor. On further analysis it was found that the synthetically produced version of the targeted lipopeptide was not pure, but in fact a mixture, and further, the targeted lipopeptide was not antigenic, but instead two novel components were found to be antigenic. Preliminary mass spectrometric investigations indicated that the unknown antigenic components are very similar in structure to each other and have a partial structure similar to the intended synthetic structure. Mass spectrometric studies at the BUSM MS Resource laboratory have included the use of MALDI and ESI Q-o-TOF and MALDI and ESI-FTMS instrumentation, as well as the enzymatic digestion of the known and unknown compounds. The structure has now been solved and a synthetic analog is being prepared. A poster and a manuscript are being readied for publication.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 从一家商业公司获得了一种脂肽的合成版本，它是最早出现在艾滋病毒感染细胞中的化合物之一，并使用来自艾滋病毒患者捐赠者的细胞来生成T细胞系。进一步分析发现，合成的靶向脂肽不是纯的，而是一种混合物，而且靶向脂肽不是抗原性的，而是发现了两个新的抗原性成分。初步的质谱学研究表明，未知的抗原组分在结构上彼此非常相似，并且具有与预期合成结构相似的部分结构。BUSM MS资源实验室的质谱学研究包括使用MALDI和ESI Q-o-TOF和MALDI和ESI-FTMS仪器，以及对已知和未知化合物的酶消化。结构现在已经解决，合成模拟正在准备中。一张海报和一份手稿正在准备出版。