INTEGRATIVE NEUROSCIENCE INITIATIVE ON ALCOHOLISM (INIA)

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。目的：使用酒精中毒的动物模型--嗜酒大鼠(P-RAGE)的体内定量神经成像来检查酒精暴露的纵向影响，同时控制随年龄发生的形态变化。方法：采用纵向设计，对两组成年P-大鼠进行结构磁共振成像。每个队列中的一些人从未接触过酒精，而另一些人则接受了酒精：三瓶装的选择，有0%、15%(或20%)、30%(或40%)几种不同的暴露方案：持续暴露；两周一轮，然后停酒两周；以及在黑暗中狂饮导致高酒精摄入量。当大鼠大约3、6、10、13、15和19个月时，重复扫描。结果：非酒精暴露组大鼠的全脑体积在15个月左右达到最大值，而体重继续增加，没有渐近线。在所测量的大脑结构中，生长并不是均匀的。在最初12个月的研究中，胼胝体面积扩大了36%，小脑面积扩大了17%，海马体面积扩大了10%；尽管脑室扩大了21%，但由于受试者之间的巨大差异，影响并不显著。影响生长速度估计的因素包括窝点效应、磁共振图像信噪比和测量误差。与人类关于老化脑组织局部体积下降的纵向报告不同，成年大鼠的几个大脑结构继续增长。酒精暴露的大鼠也显示出大脑的增长，但这种增长被减弱了，特别是在穹窿体部。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Aims: Use in vivo quantitative neuroimaging of alcohol-preferring rat (P-rat), an animal model of alcoholism, to examine longitudinal effects of alcohol exposure, while controlling for morphological changes that occur with age. Methods: Structural MRI was acquired in a longitudinal design on two cohorts of adult P-rats. Some of each cohort were never exposed to alcohol, while others received alcohol: a three-bottle choice with 0%, 15% (or 20%), and 30 % (or 40%) several different exposure schemes: continuous exposure; cycles of 2 weeks on followed by 2 weeks off alcohol; and binge drinking in the dark leading to high levels of alcohol consumption. Scanning was repeated when rats were approximately 3, 6, 10, 13, 15, and 19 months. Results: The non-alcohol exposed rats showed growth in whole brain volume that reached maximal levels by about 15 months, whereas body weight continued its gain without asymptote. Growth was not uniform across brain structures measured. Over the initial 12 months of the study, corpus callosum area expanded 36%, cerebellum 17%, and hippocampus 10%; although the ventricles enlarged 21%, the effect was not significant because of substantial between-subject variance. Factors affecting growth rate estimates included litter effects, MR image signal-to-noise ratio, and measurement error. Unlike longitudinal human reports of regional volume declines in aging brain tissue, several brain structures in adult rats continued growing. The alcohol-exposed rats also showed brain growth but this was attenuated, particularly in the corpus callosum.