EXAFS ANALYSIS OF METAL-NUCLEIC ACID COMPLEXES

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。这项建议探索了EXAFS在阐明核酸-金属络合物中的金属-离子结合部位的应用。DNA四链体、核酶和脱氧核酶是本项目要考虑的金属-核复合体的例子。被检测的金属包括K+、Pb2和Sr2。我们在Pb2-DNA四链结构上获得的初步结果是非常有希望的，表明EXAFS在这一结构生物学领域具有潜在的应用前景。到目前为止，将EXAFS应用于核酸的工作还很少，我们的研究应该有助于将这种方法作为一种重要的结构工具。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This proposal explores the application of EXAFS to elucidating metal-ion binding sites in nucleic acid-metal complexes. DNA quadruplexes, ribozymes and deoxyribozymes are examples of the metal-nucleic complexes to be considered in this project. Metals to be examined include K+, Pb+2 and Sr+2. Our preliminary results obtained on a Pb+2-DNA quadruplex are very promising and indicate the potential utility of EXAFS in this area of structural biology. There has been very little work to date applying EXAFS to nucleic acids and our studies should help establish this method as an important structural tool.