STRUCTURAL STUDIES OF METALLOPROTEINS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。金属在许多蛋白质的功能中起着不可或缺的作用。这些金属蛋白的结构测定是阐明金属介导其生物过程的分子机制的基础。本课题主要从三个方面对金属酶进行结构研究：(1)参与醋酸和甲胺生成甲基的产甲烷蛋白的结构研究；(2)新型金属蛋白酶的结构研究；(3)蛋白质氧传感器的结构研究。每个推力区域的许多蛋白质已经结晶，项目目前正处于不同的阶段，以解决它们的结构。未来研究的重点将是利用在SSRL束线上收集的MAD和MIR数据完成正在进行的结构确定，并确定这些蛋白质的抑制剂和中间复合物的结构，以阐明与机制和配体结合相关的问题。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Metals play an integral role in the function of many proteins. The structural determination of these metalloproteins is fundamental for elucidating the molecular mechanisms by which metals mediate their biological processes. This proposal focuses on structural studies of metalloenzymes in three major areas: (1) the structural determination of methanogenic proteins involved in generating the methyl group from acetate and methylamines, (2) the structural determination of novel metalloproteases, and (3) the structural determination of protein oxygen sensors. A number of proteins in each thrust area have been crystallized and projects are currently at various stages towards their structure solution. The focus of future studies will be to complete ongoing structural determinations using MAD and MIR data collected on SSRL beamlines and to determine the structures of inhibitor and intermediate complexes of these proteins to clarify issues related to mechanism and ligand binding.