COLLECTION OF PROTEIN CRYSTALLOGRAPHIC DATA

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。Syrx是一家总部位于圣地亚哥的结构蛋白质组公司，从事基于结构的药物设计。几个微生物蛋白质的目标已经在Syrrx结晶，并用单色X射线进行了表征。为了使这些蛋白质相态，我们已经用Se-Met替代生长和结晶了这些蛋白质，并正在寻找疯狂的束流时间。我们将在光束线9-2收集蛋白质晶体X射线衍射数据。我们将使用标准的光束线操作程序和设备。所有晶体都将被带到SSRL预冻，并将在实验结束时从现场移除

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Syrrx is a structural proteomics company based in San Diego doing structure-based drug design. Several targets of microbial proteins have been crystallized at Syrrx, characterized with monochromatic X-rays. In order to phase these we have grown and crystallized these proteins with Se-Met substitution and are seeking MAD beam time. We will be collecting protein crystal X-ray diffraction data at beamline 9-2. We will be using standard beamline operating procedures and equipment. All crystals are being brought to SSRL pre-frozen and will be removed from the site at the end of the experiment