HIGH RESOLUTION STRUCTURAL STUDIES ON MACROMOLECULAR CRYSTALS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。将为七个不同的结晶学项目收集同步辐射X射线衍射数据。(1)研究突变的葡萄糖异构酶体系、无底物、底物结合和活性中心，以获得超高分辨结构。这项工作将与计划于2001年11月在劳埃朗之万研究所对所有三个样品进行的束时间中子衍射研究相协调，我们将在该研究中阐明酶机制。(2)中分辨率同步加速器数据(&gt；3？)将在碱性成纤维细胞生长因子/DNA复合体上收集，由于大的单位细胞边缘，该复合体迄今未能进行实验室研究。(3)将进行MAD研究，以获得C1Titin 2-结构域片段的结构；(4)将马血清白蛋白用于研究微重力的影响，以提高当前结构的分辨率。(5)最后将对尘螨变应原Der f2进行结构研究，(6)对RNA十二聚体进行结构研究，(7)对RNA假结进行结构研究。每个系统都有工业、生物医学和马血清白蛋白研究的基本重要性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Synchrotron X-ray diffraction data will be collected for seven different crystallographic projects. (1) A mutant glucose isomerase system, substrate free, substrate bound and active site will be investigated to obtain ultra-high resolution structures. This effort will be coordinated with a neutron diffraction study on all three samples scheduled for beamtime at the Institute Laue Langevin in November 2001 in which we will elucidate the enzymatic mechanism. (2) Medium resolution synchrotron data (>3 ¿¿ will be collected on a bFGF/DNA complex that has thus far eluded laboratory studies due to the large unit cell edge. (3) A MAD study will be carried out to obtain the structure of the C1, titin 2-domain fragment, (4) Equine serum albumin will be used for studying the effects of microgravity and to improve the resolution of the current structure. (5) Finally structural studies will be carried out on Der f2, a dust mite allergen (6) on an RNA dodecamer, and (7) on RNA pseudoknot. Each system has industrial, biomedical and in the case of the equine serum albumin study, fundamental importance.