STRUCTURAL STUDIES OF NUCLEIC ACID-BINDING PROTEINS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。蛋白质对核酸的识别和结合在细胞生物化学中起着重要作用。我们有兴趣研究特异性和非特异性蛋白质-核酸相互作用的结构基础。我们正在研究非特异性DNA结合，通过结构研究阐明两种不同的DNA外切核酸酶的持续合成机制。特定的蛋白质-DNA相互作用将使用果蝇发育蛋白质与DNA复合物进行研究。为了更好地了解蛋白质-RNA相互作用的结构基础，我们正在研究细菌DEAD盒RNA解旋酶。这些结构的研究将提供一个更好的理解的结构特征，管理蛋白质-核酸相互作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recognition and binding of nucleic acids by proteins plays a fundamental role in cellular biochemistry. We are interested in studying the structural basis for specific and non-specific protein-nucleic acid interactions. We are investigating non-specific DNA-binding by elucidating the mechanisms of processivity of two distinct DNA exonucleases through structural studies. Specific protein-DNA interactions will be investigated using a Drosophila developmental protein in complex with DNA. In order to gain better insight into the structural basis of protein-RNA interactions, we are looking at a bacterial DEAD-box RNA helicase. These structural studies will provide a better understanding of the structural characteristics that govern protein-nucleic acid interactions.